Recombinant
RabMAb

Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free (ab225900)

Overview

  • Product name
    Anti-LEF1 antibody [EPR2029Y] - BSA and Azide free
    See all LEF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2029Y] to LEF1 - BSA and Azide free
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LEF1 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • IHC-P: Rat spleen tissue.
  • General notes

    ab225900 is a PBS-only buffer format of ab137872. Please refer to ab137872 for recommended dilutions, protocols, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.

We don’t recommend this antibody for mouse in Western Blot. In our hands an extra band was observed in mouse tissue lysates.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
  • Tissue specificity
    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.
  • Sequence similarities
    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.
  • Domain
    Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
  • Cellular localization
    Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586H0919 antibody
    • FLJ46390 antibody
    • LEF 1 antibody
    • LEF-1 antibody
    • Lef1 antibody
    • LEF1_HUMAN antibody
    • Lymphoid enhancer binding factor 1 antibody
    • Lymphoid enhancer-binding factor 1 antibody
    • T cell specific transcription factor 1 alpha antibody
    • T cell-specific transcription factor 1-alpha antibody
    • TCF 1 alpha antibody
    • TCF1 alpha antibody
    • TCF1-alpha antibody
    • TCF10 antibody
    • TCF1alpha antibody
    • TCF7L3 antibody
    • Transcription factor T cell specific 1 alpha antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded rat spleen with purified ab137872 at a working dilution of 1/500. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137872).

References

ab225900 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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