Anti-LHX3 antibody [EPR3344(2)] (ab124697)

Overview

• Product nameAnti-LHX3 antibody [EPR3344(2)]See all LHX3 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3344(2)] to LHX3
• Tested applicationsWB, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide, corresponding to residues from Human LHX3.

• Positive control293T and Jurkat cell lysates, Human brain and fetal lung tissue lysates; permeabilized Jurkat cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 6.84 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3344(2)
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • LIM

Applications

Target

• FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
• Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
• Sequence similaritiesContains 1 homeobox DNA-binding domain.
  Contains 2 LIM zinc-binding domains.
• DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9UBR4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8022 Human
  • Entrez Gene: 16871 Mouse
  • Entrez Gene: 170671 Rat
  • Omim: 600577 Human
  • SwissProt: Q9UBR4 Human
  • SwissProt: P50481 Mouse
  • Unigene: 148427 Human
  • Unigene: 386765 Mouse

  • Unigene: 198623 Rat

  see all

• Alternative names
  CPHD 3 antibodyCPHD3 antibodyDKFZp762A2013 antibody

  LHX 3 antibodyLHX3 antibodyLHX3_HUMAN antibodyLIM 3 antibodyLIM homeobox 3 antibodyLIM homeobox gene 3 antibodyLIM homeobox protein 3 antibodyLIM/homeobox protein Lhx3 antibodyLIM/homeodomain protein LHX3 antibodyLim3 antibodyM2 LHX3 antibodymLim-3 antibodymLIM3 antibodyP LIM antibody

  see all

Anti-LHX3 antibody [EPR3344(2)] images

• 

  Western blot - Anti-LHX3 antibody [EPR3344(2)] (ab124697)
  All lanes : Anti-LHX3 antibody [EPR3344(2)] (ab124697) at 1/1000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : Human brain tissue lysate
  Lane 3 : Jurkat cell lysate
  Lane 4 : Human fetal lung lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-Rabbit HRP at 1/2000 dilution
  
  Predicted band size : 43 kDa
  
• 

  Flow Cytometry - Anti-LHX3 antibody [EPR3344(2)] (ab124697)
  ab124697 at 1/10 dilution, staining LHX3 in permeabilized Jurkat cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
• 

  Other-Anti-LHX3 antibody [EPR3344(2)](ab124697)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D