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  • Product nameAnti-LHX3 antibody
    See all LHX3 primary antibodies
  • Description
    Rabbit polyclonal to LHX3
  • Specificityab26025 recognises LHX3.
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: EKRLKKDAGR QRWGQYFRNM KRSRGGSKSD KDSVQEGQDS DAEVSFPDEP, corresponding to an sequence within internal sequence amino acids 217-266 of Human LHX3

  • Positive control
    • Jurkat cell lysate, human lung tissue, human heart tissue



Our Abpromise guarantee covers the use of ab26025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPHD 3 antibody
    • CPHD3 antibody
    • DKFZp762A2013 antibody
    • LHX 3 antibody
    • LHX3 antibody
    • LHX3_HUMAN antibody
    • LIM 3 antibody
    • LIM homeobox 3 antibody
    • LIM homeobox gene 3 antibody
    • LIM homeobox protein 3 antibody
    • LIM/homeobox protein Lhx3 antibody
    • LIM/homeodomain protein LHX3 antibody
    • Lim3 antibody
    • M2 LHX3 antibody
    • mLim-3 antibody
    • mLIM3 antibody
    • P LIM antibody
    see all

Anti-LHX3 antibody images

  • Anti-LHX3 antibody (ab26025) at 1.25 µg/ml

    Predicted band size : 44 kDa
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded heart tissue. Cells with positive label (as indicated by the arrows) are myocardial cells. Magnification 400X.

References for Anti-LHX3 antibody (ab26025)

ab26025 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). I hop...

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