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Overview

  • Product nameAnti-LHX3 antibodySee all LHX3 primary antibodies ...
  • Description
    Rabbit polyclonal to LHX3
  • Specificityab26025 recognises LHX3.
  • Tested applicationsELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: EKRLKKDAGR QRWGQYFRNM KRSRGGSKSD KDSVQEGQDS DAEVSFPDEP, corresponding to an sequence within internal sequence amino acids 217-266 of Human LHX3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlJurkat cell lysate, human lung tissue, human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab26025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/312500.
WB WB: Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P IHC-P: Use at an assay dependent concentration.

Target

  • FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9UBR4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CPHD 3 antibodyCPHD3 antibodyDKFZp762A2013 antibody
        LHX 3 antibodyLHX3 antibodyLHX3_HUMAN antibodyLIM 3 antibodyLIM homeobox 3 antibodyLIM homeobox gene 3 antibodyLIM homeobox protein 3 antibodyLIM/homeobox protein Lhx3 antibodyLIM/homeodomain protein LHX3 antibodyLim3 antibodyM2 LHX3 antibodymLim-3 antibodymLIM3 antibodyP LIM antibodyP-LIM antibody
      see all

    Anti-LHX3 antibody images

    • Western blot - LHX3 antibody (ab26025)
      Western blot - LHX3 antibody (ab26025)
      Anti-LHX3 antibody (ab26025) at 1.25 µg/ml

      Predicted band size : 44 kDa
    • Immunohistochemistry (Paraffin-embedded sections) - LHX3 antibody (ab26025)
      Immunohistochemistry (Paraffin-embedded sections) - LHX3 antibody (ab26025)
      ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
    • Immunohistochemistry (Paraffin-embedded sections) - LHX3 antibody (ab26025)
      Immunohistochemistry (Paraffin-embedded sections) - LHX3 antibody (ab26025)
      ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded heart tissue. Cells with positive label (as indicated by the arrows) are myocardial cells. Magnification 400X.

    References for Anti-LHX3 antibody (ab26025)

    ab26025 has not yet been referenced specifically in any publications.

    Publishing research using ab26025 ? Please let us know so that we can cite the reference in this datasheet

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    What was the lysis buffer used to prepare the samples run on the Western blot?

    Thank you for your enquiry. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). I hope t...

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