Overview

  • Product nameAnti-LHX3 antibodySee all LHX3 primary antibodies ...
  • Description
    Rabbit polyclonal to LHX3
  • Specificityab26025 recognises LHX3.
  • Tested applicationsELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide: EKRLKKDAGR QRWGQYFRNM KRSRGGSKSD KDSVQEGQDS DAEVSFPDEP, corresponding to an sequence within internal sequence amino acids 217-266 of Human LHX3

  • Positive control
    • Jurkat cell lysate, human lung tissue, human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab26025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
  • Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q9UBR4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CPHD 3 antibody
    • CPHD3 antibody
    • DKFZp762A2013 antibody
    • LHX 3 antibody
    • LHX3 antibody
    • LHX3_HUMAN antibody
    • LIM 3 antibody
    • LIM homeobox 3 antibody
    • LIM homeobox gene 3 antibody
    • LIM homeobox protein 3 antibody
    • LIM/homeobox protein Lhx3 antibody
    • LIM/homeodomain protein LHX3 antibody
    • Lim3 antibody
    • M2 LHX3 antibody
    • mLim-3 antibody
    • mLIM3 antibody
    • P LIM antibody
    • P-LIM antibody
    see all

Anti-LHX3 antibody images

  • Anti-LHX3 antibody (ab26025) at 1.25 µg/ml

    Predicted band size : 44 kDa
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26025 at a concentration of 6.0 µg/ml staining human LHX3 by Immunohistochemistry in paraffin embedded heart tissue. Cells with positive label (as indicated by the arrows) are myocardial cells. Magnification 400X.

References for Anti-LHX3 antibody (ab26025)

ab26025 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). I hop...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"