Overview
- Product nameLIF Human ELISA KitSee all LIF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range8.23 pg/ml - 6000 pg/ml
- Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.41 84% - 103% Serum 94.26 83% - 106% Plasma 93.64 82% - 102% - Species reactivityReacts with: Human
- Product overview
LIF (Leukocyte inhibitory factor) is produced by various fibroblast cell lines, antigen-stimulated alloreactive T-lymphocytes, mitogen-activated spleen cells, and Krebs and Ehrlich ascites cells. It is also produced by monocytes after cell activation. This factor inhibits the random and directed migration of polymorphonuclear (PMN) leukocytes. LIF also induces specific granule secretion by PMNs and potentiates many responses mediated by the chemotactic compound fMLP (formyl-methionyl-leucylphenylalanine).
ab100582 LIF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIF in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Human LIF coated on a 96-well plate. Standards and samples are pipetted into the wells and LIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Notes
Optimization may be required with urine samples.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody LIF: biotinylated anti-human LIF 2 vials HRP-Streptavidin Concentrate 320x concentrated HRP-conjugated streptavidin 1 x 200µl HRP-Streptavidin Concentrate 320x concentrated HRP-conjugated streptavidin 1 x 200µl LIF Human Standard (recombinant) 2 vials LIF Microplate (12 strips x 8 wells) coated with anti-human LIF 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml - Research Areas
Target information above from: UniProt accession
P15018
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CDFCholinergic differentiation factorD factor
- DIADifferentiation inhibitory activityDifferentiation stimulating factorDifferentiation-stimulating factorEmfilerminHepatocyte stimulating factor IIIHILDAHuman interleukin in DA cellsLeukemia inhibitory factorLIFLIF_HUMANMelanoma derived LPL inhibitorMelanoma-derived LPL inhibitorMLPLI
see all
- Entrez Gene: 3976 Human
- Omim: 159540 Human
- SwissProt: P15018 Human
- Unigene: 2250 Human
Applications
Our Abpromise guarantee covers the use of ab100582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
LIF Human ELISA Kit images
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ELISA - LIF Human ELISA Kit (ab100582)
Protocols
References for LIF Human ELISA Kit (ab100582)
ab100582 has not yet been referenced specifically in any publications.
