Anti-LIM Kinase 1 antibody [3G3] (ab119084)

Overview

  • Product nameAnti-LIM Kinase 1 antibody [3G3]See all LIM Kinase 1 primary antibodies ...
  • Description
    Mouse monoclonal [3G3] to LIM Kinase 1
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human LIM Kinase 1 produced in HEK293T cells (NP_002305) .

  • Positive control
    • HEK293T cells transfected with LIM Kinase 1, COS7 cells transiently transfected with LIM Kinase 1
  • General notesConcentration 0.5~1.0 mg/ml (Lot Dependent)

Properties

Applications

Our Abpromise guarantee covers the use of ab119084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000. Predicted molecular weight: 73 kDa.
ICC/IF 1/100.

Target

  • FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localizationCytoplasm. Cell projection > growth cone.
  • Target information above from: UniProt accession P53667 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.1.37 antibody
    • LIM domain containing protein kinase antibody
    • LIM domain kinase 1 antibody
    • LIM motif containing protein kinase antibody
    • LIMK 1 antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody [3G3] images

  • All lanes : Anti-LIM Kinase 1 antibody [3G3] (ab119084) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected the pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected the pCMV6-ENTRY LIM Kinase 1

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 73 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218058)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.

References for Anti-LIM Kinase 1 antibody [3G3] (ab119084)

ab119084 has not yet been referenced specifically in any publications.

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