RabMAb

Anti-LIM Kinase 1 antibody [EPR912] (ab108507)

Overview

  • Product nameAnti-LIM Kinase 1 antibody [EPR912]See all LIM Kinase 1 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR912] to LIM Kinase 1
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminal region of Human LIM Kinase 1.

  • Positive control
    • U-87 MG, SH SY5Y and Ramos cell lysates.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab108507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • Cellular localizationCytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 antibody [EPR912] images

    • All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa

    References for Anti-LIM Kinase 1 antibody [EPR912] (ab108507)

    ab108507 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"