Anti-LIM Kinase 1 antibody [EPR912] (ab108507)

Overview

• Product nameAnti-LIM Kinase 1 antibody [EPR912]See all LIM Kinase 1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR912] to LIM Kinase 1
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  A synthetic peptide corresponding to residues near the N terminal region of Human LIM Kinase 1.

• Positive controlU-87 MG, SH SY5Y and Ramos cell lysates.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR912
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • LIM

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
• Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
• Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
• Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
  Contains 2 LIM zinc-binding domains.
  Contains 1 PDZ (DHR) domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated.
  Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
  Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
• Cellular localizationCytoplasm. Cell projection > growth cone.

Target information above from: UniProt accession P53667 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3984 Human
  • Entrez Gene: 3984 Human
  • Entrez Gene: 16885 Mouse
  • Omim: 601329 Human
  • SwissProt: P53667 Human
  • SwissProt: P53667 Human
  • SwissProt: P53668 Mouse
  • Unigene: 647035 Human

  • Unigene: 15409 Mouse

  see all

• Alternative names
  EC 2.7.1.37 antibodyLIM domain containing protein kinase antibodyLIM domain kinase 1 antibody

  LIM motif containing protein kinase antibodyLIMK 1 antibodyLIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody

  see all

Anti-LIM Kinase 1 antibody [EPR912] images

• 

  Western blot - LIM Kinase 1 antibody [EPR912] (ab108507)
  All lanes : Anti-LIM Kinase 1 antibody [EPR912] (ab108507) at 1/1000 dilution
  
  Lane 1 : U-87 MG cell lysate
  Lane 2 : SH SY5Y cell lysate
  Lane 3 : Ramos cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 73 kDa