You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab10561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localizationCytoplasm. Cell projection > growth cone.

    • Target information above from: UniProt accession P53667 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      EC 2.7.1.37 antibodyLIM domain containing protein kinase antibodyLIM domain kinase 1 antibody
      LIM motif containing protein kinase antibodyLIMK 1 antibodyLIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody images

  • Western blot - LIM Kinase 1 antibody (ab10561)
    Western blot - LIM Kinase 1 antibody (ab10561)


    Predicted band size : 73 kDa


    Rabbit polyclonal to LIM kinase (1/500) - ab10561. 18 ug of mouse brain lysate loaded. Expected mol weight : 73 kD. Bands observed at 75 kD which we believe represents LIM kinase (doublet - may indicate modification such as phosphorylation) and 25 kD (we do not know the identity of this band). Secondary ab - Goat polyclonal to rabbit IgG (HRP) preabsorbed

References for Anti-LIM Kinase 1 antibody (ab10561)

ab10561 has not yet been referenced specifically in any publications.

Publishing research using ab10561 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab10561 Submit a Question for ab10561

There are currently no Abreviews or Questions for ab10561.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"