Anti-LIM Kinase 1 antibody (ab39641)

Overview

• Product nameAnti-LIM Kinase 1 antibodySee all LIM Kinase 1 primary antibodies ...
• Description
  Rabbit polyclonal to LIM Kinase 1
• Specificityab39641 detects endogenous levels of total LIM Kinase 1 protein.
• Tested applicationsICC/IF, WB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.

• Positive controlA2780 and NIH/3T3 cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • LIM

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
• Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
• Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
• Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
  Contains 2 LIM zinc-binding domains.
  Contains 1 PDZ (DHR) domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated.
  Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
  Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
• Cellular localizationCytoplasm. Cell projection > growth cone.

Target information above from: UniProt accession P53667 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3984 Human
  • Entrez Gene: 3984 Human
  • Entrez Gene: 16885 Mouse
  • Entrez Gene: 65172 Rat
  • Omim: 601329 Human
  • SwissProt: P53667 Human
  • SwissProt: P53667 Human
  • SwissProt: P53668 Mouse

  • SwissProt: P53669 Rat
  • Unigene: 647035 Human
  • Unigene: 15409 Mouse
  • Unigene: 11250 Rat

  see all

• Alternative names
  EC 2.7.1.37 antibodyLIM domain containing protein kinase antibodyLIM domain kinase 1 antibody

  LIM motif containing protein kinase antibodyLIMK 1 antibodyLIMK 1 antibodyLIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody

  see all

Anti-LIM Kinase 1 antibody images

• 

  Western blot - LIM Kinase 1 antibody (ab39641)
  All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution
  
  Lane 1 : NIH/3T3 cell extracts
  Lane 2 : A2780 cell extracts
  
  
  Predicted band size : 73 kDa
  Observed band size : 73 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - LIM Kinase 1 antibody (ab39641)Image from Montani L et al, J Biol Chem. 2009 Apr 17;284(16):10793-807. Epub 2009 Feb 9, Fig 5, DOI 10.1074/jbc.M808297200.
  ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.
  
  Scale bar = 10µm.