Anti-LIM Kinase 1 antibody (ab39641)
- Product nameAnti-LIM Kinase 1 antibodySee all LIM Kinase 1 primary antibodies ...
- DescriptionRabbit polyclonal to LIM Kinase 1
- Specificityab39641 detects endogenous levels of total LIM Kinase 1 protein.
- Tested applicationsICC/IF, WB, ELISA more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.
- Positive controlA2780 and NIH/3T3 cells.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab39641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 (PMID 19208621).
WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
- Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
- Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
- Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
- Cellular localizationCytoplasm. Cell projection > growth cone.
- EC 22.214.171.124 antibodyLIM domain containing protein kinase antibodyLIM domain kinase 1 antibody
- LIM motif containing protein kinase antibodyLIMK 1 antibodyLIMK 1 antibodyLIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody
Anti-LIM Kinase 1 antibody images
All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : A2780 cell extracts
Predicted band size : 73 kDa
Observed band size : 73 kDa
ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.Scale bar = 10µm.
References for Anti-LIM Kinase 1 antibody (ab39641)
This product has been referenced in:
- Tondeleir D et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). Read more (PubMed: 22448045) »
- Montani L et al. Neuronal Nogo-A Modulates Growth Cone Motility via Rho-GTP/LIMK1/Cofilin in the Unlesioned Adult Nervous System. J Biol Chem 284:10793-807 (2009). WB, ICC/IF ; Rat . Read more (PubMed: 19208621) »