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Overview

  • Product nameAnti-LIM Kinase 1 antibodySee all LIM Kinase 1 primary antibodies ...
  • Description
    Mouse monoclonal to LIM Kinase 1
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: ADPDYLPRTM DFGLNVRGFL DRYCPPNCPP SFFPITVRCC DLDPEKRPSF VKLEHWLETL RMHLAGHLPL GPQLEQLDRG FWETYRRGES GLPAHPEVPD, corresponding to amino acids 548-647 of human LIM Kinase 1 protein (NP_002305) with a proprietary tag.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHepG2, SW-13 and NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA ELISA: Use at an assay dependent dilution.

Target

  • FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localizationCytoplasm. Cell projection > growth cone.

    • Target information above from: UniProt accession P53667 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      EC 2.7.1.37 antibodyLIM domain containing protein kinase antibodyLIM domain kinase 1 antibody
      LIM motif containing protein kinase antibodyLIMK 1 antibodyLIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody
    see all

Anti-LIM Kinase 1 antibody images

  • Western blot - LIM Kinase 1 antibody (ab87971)
    Western blot - LIM Kinase 1 antibody (ab87971)
    Anti-LIM Kinase 1 antibody (ab87971) at 1/500 dilution + recombinant protein fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Western blot - LIM Kinase 1 antibody (ab87971)
    Western blot - LIM Kinase 1 antibody (ab87971)
    Anti-LIM Kinase 1 antibody (ab87971) at 1/500 dilution + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • Western blot - LIM Kinase 1 antibody (ab87971)
    Western blot - LIM Kinase 1 antibody (ab87971)
    Anti-LIM Kinase 1 antibody (ab87971) at 1/500 dilution + SW-13 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
  • Western blot - LIM Kinase 1 antibody (ab87971)
    Western blot - LIM Kinase 1 antibody (ab87971)
    Anti-LIM Kinase 1 antibody (ab87971) at 1/500 dilution + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-LIM Kinase 1 antibody (ab87971)

ab87971 has not yet been referenced specifically in any publications.

Publishing research using ab87971 ? Please let us know so that we can cite the reference in this datasheet

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