Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
Key features and details
- Rabbit polyclonal to LIM Kinase 1 (phospho T508)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIM Kinase 1 (phospho T508) antibody
See all LIM Kinase 1 primary antibodies -
Description
Rabbit polyclonal to LIM Kinase 1 (phospho T508) -
Host species
Rabbit -
Specificity
This antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human LIM Kinase 1 (phospho T508).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. -
Tissue specificity
Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle. -
Involvement in disease
Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
Cellular localization
Cytoplasm. Cell projection > growth cone. - Information by UniProt
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Database links
- Entrez Gene: 3984 Human
- Omim: 601329 Human
- SwissProt: P53667 Human
- Unigene: 647035 Human
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Alternative names
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
Images
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ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution
Lane 1 : Extract of COLO cells + peptide.
Lane 2 : Extract of COLO cells.
Lane 3 : Extract of MCF7 cells.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508). -
ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab38508 has been referenced in 16 publications.
- Zhu YT et al. Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after stroke. Neural Regen Res 16:2512-2520 (2021). PubMed: 33907042
- Lee KY et al. Infantile spasms-linked Nedd4-2 mediates hippocampal plasticity and learning via cofilin signaling. EMBO Rep 22:e52645 (2021). PubMed: 34342389
- Baharani A et al. A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 33108594
- Ravindran S et al. BDNF Induced Translation of Limk1 in Developing Neurons Regulates Dendrite Growth by Fine-Tuning Cofilin1 Activity. Front Mol Neurosci 12:64 (2019). PubMed: 30949027
- Lee M et al. Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. J Cell Biol 218:2659-2676 (2019). PubMed: 31270137