Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)

Overview

  • Product nameAnti-LIM Kinase 1 (phospho T508) antibodySee all LIM Kinase 1 primary antibodies ...
  • Description
    Rabbit polyclonal to LIM Kinase 1 (phospho T508)
  • SpecificityThis antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat.
  • Tested applicationsWB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from Human LIM Kinase around the phosphorylation site of threonine 508.

  • Positive control
    • IHC-P: Human breast carcinoma tissue slides. WB: COLO and MCF7 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab38508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
ICC/IF
  • Application notesELISA: 1/10000.
    ICC/IF: 1/100 (pmid 19208621).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • Cellular localizationCytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Anti-LIM Kinase 1 (phospho T508) antibody images

    • ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

      Left image : untreated.

      Right image : treated with phosphopeptide.

    • All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution

      Lane 1 : Extract of COLO cells + peptide.
      Lane 2 : Extract of COLO cells.
      Lane 3 : Extract of MCF7 cells.

      Lysates/proteins at 30 µg per lane.

      Secondary
      Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)

      Predicted band size : 73 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    • ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.

    References for Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)

    This product has been referenced in:
    • Loubet D  et al. Neuritogenesis: the prion protein controls ß1 integrin signaling activity. FASEB J 26:678-90 (2012). WB ; Mouse . Read more (PubMed: 22038049) »
    • Tondeleir D  et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics 11:255-71 (2012). WB . Read more (PubMed: 22448045) »

    See all 6 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (Brain)
    Loading amount 20 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 27 2011

    Thank you for your patience. I have been in contact with the originator of this antibody, and below is the detailed antigen retrieval protocol. The method of antigen retrieval was used for IHC-P as following: Deparaffinization 1. Incubate slide a...

    Read More

    Thank you for your enquiry. From data of recent testing, this antibody recognizes both LIMK1 and LIMK2 from human, mouse, and rat. I have clarified this on the datasheet; should you have further questions please don't hesitate to contact me,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"