Anti-LIMK1 antibody (ab95186)

Overview

• Product nameAnti-LIMK1 antibodySee all LIMK1 primary antibodies ...
• Description
  Rabbit polyclonal to LIMK1
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide corresponding to a region within C terminal amino acids 597-647 of Human LIMK1 (NP_002305.1)

• Positive controlHeLa whole cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab95186 was affinity purified using an epitope specific to LIMK1 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Regulation

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Growth Cone

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
• Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
• Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
• Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
  Contains 2 LIM zinc-binding domains.
  Contains 1 PDZ (DHR) domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated.
  Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
  Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
• Cellular localizationCytoplasm. Cell projection > growth cone.

Target information above from: UniProt accession P53667 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3984 Human
  • Omim: 601329 Human
  • SwissProt: P53667 Human
  • Unigene: 647035 Human

• Alternative names
  EC 2.7.11.1 antibodyLIM domain kinase 1 antibodyLIM motif-containing protein kinase antibody

  LIMK antibodyLIMK-1 antibodylimk1 antibodyLIMK1_HUMAN antibody

  see all

Anti-LIMK1 antibody images

• 

  Western blot - LIMK1 antibody (ab95186)
  All lanes : Anti-LIMK1 antibody (ab95186) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  
  developed using the ECL technique
  
  Predicted band size : 73 kDa
  
  
  Exposure time : 30 seconds
• 

  Immunoprecipitation - LIMK1 antibody (ab95186)
  ab95186 at 3 µg/mg precipitating LIMK1 from HeLa whole cell lysate.
  Lane 1; HeLa whole cell lysate. Lane 2; Control IgG. 1mg of lysate used for IP and 20% of IP loaded.
  Detection utilised ab95186 at 0.4 µg/ml followed by ECL with a 5 second exposure.