LIMP II Human ELISA Kit (ab113336)
- Product nameLIMP II Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range10.24 pg/ml - 2500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.06 74% - 107% Serum 83 74% - 92% Plasma 79.65 71% - 86%
- Species reactivityReacts with: Human
- Product overview
ab113336 Human LIMP II ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LIMP II in serum, plasma, cell culture supernatants . This assay employs an antibody specific for human LIMP II coated on a 96-well plate. Standards and samples are pipetted into the wells and LIMP II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman LIMP II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIMP II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent B Item E 1 x 15ml Assay Diluent C Item L 1 x 30ml Detection Antibody Item F 2 vials LIMP II Microplate (12 strips x 8 wells) coated with anti-Human LIMP II Item A 1 unit LIMP II Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Note=Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
- 85 kDa lysosomal membrane sialoglycoproteinCD36CD36 antigen-like 2
- LGP85LIMP IILysosome membrane protein 2Lysosome membrane protein IIScarb2Scavenger receptor class B member 2SCRB2_HUMAN
Our Abpromise guarantee covers the use of ab113336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for LIMP II Human ELISA Kit (ab113336)
ab113336 has not yet been referenced specifically in any publications.