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Overview

  • Product nameLIMP II Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    10.24 pg/ml - 2500 pg/ml
  • Recovery

    86 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.06 74% - 107%
    Serum 83 74% - 92%
    Plasma 79.65 71% - 86%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab113336 Human LIMP II ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human LIMP II in serum, plasma, cell culture supernatants . This assay employs an antibody specific for human LIMP II coated on a 96-well plate. Standards and samples are pipetted into the wells and LIMP II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman LIMP II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIMP II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionAct as a lysosomal receptor for glucosylceramidase (GBA) targeting.
  • Involvement in diseaseDefects in SCARB2 are the cause of progressive myoclonic epilepsy type 4 with or without renal failure (EPM4) [MIM:254900]. An autosomal recessive progressive myoclonic epilepsy associated with renal failure in some cases. Cognitive function is preserved. Myoclonus is a brief, involuntary twitching of a muscle or a group of muscles. Cognitive function is preserved.
    Note=Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • Sequence similaritiesBelongs to the CD36 family.
  • Cellular localizationLysosome membrane.

    • Target information above from: UniProt accession Q14108 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        85 kDa lysosomal membrane sialoglycoproteinCD36CD36 antigen-like 2
        LGP85LIMP IILysosome membrane protein 2Lysosome membrane protein IIScarb2Scavenger receptor class B member 2SCRB2_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab113336 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      LIMP II Human ELISA Kit images

      • ELISA - LIMP II Human ELISA Kit (ab113336)
        ELISA - LIMP II Human ELISA Kit (ab113336)
        Standard curve
      • ELISA - LIMP II Human ELISA Kit (ab113336)
        ELISA - LIMP II Human ELISA Kit (ab113336)
        Standard curve

      Protocols

      References for LIMP II Human ELISA Kit (ab113336)

      ab113336 has not yet been referenced specifically in any publications.

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