Recombinant Anti-LIPF antibody [EPR5711(B)] (ab124831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5711(B)] to LIPF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-LIPF antibody [EPR5711(B)]
See all LIPF primary antibodies -
Description
Rabbit monoclonal [EPR5711(B)] to LIPF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human LIPF aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- Human stomach and Mouse small intestine lysates; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5711(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(heat up to 98 degrees C, below boiling, and then let cool for 10-20 min). |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min). |
Target
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Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8513 Human
- Entrez Gene: 67717 Mouse
- Omim: 601980 Human
- SwissProt: P07098 Human
- SwissProt: Q9CPP7 Mouse
- Unigene: 523130 Human
- Unigene: 329816 Mouse
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Alternative names
- Gastric lipase antibody
- Gastric triacylglycerol lipase antibody
- gL antibody
see all
Images
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All lanes : Anti-LIPF antibody [EPR5711(B)] (ab124831) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Mouse small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
ab124831, at 1/250 dilution, staining LIPF in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124831 has not yet been referenced specifically in any publications.