Overview

  • Product name
  • Description
    Rabbit polyclonal to LMX1b
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human, LMX1b isoform) conjugated to KLH. Protein length 372 aa. First peptide is close to the N-terminal between 1-60 aa. The second and third peptides are close to the C-terminal between 241-372 aa.

  • Positive control
    • Induced culture of E.coli (one shot Top10 competent cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab82835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/70000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
    • Tissue specificity
      Expressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.
    • Involvement in disease
      Defects in LMX1B are the cause of nail-patella syndrome (NPS) [MIM:161200]; also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia.
    • Sequence similarities
      Contains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • LIM homeo box transcription factor 1 beta antibody
      • LIM homeobox transcription factor 1 beta antibody
      • LIM homeobox transcription factor 1-beta antibody
      • LIM-homeobox protein 1.2 antibody
      • LIM/homeobox protein 1.2 antibody
      • LIM/homeobox protein LMX1B antibody
      • LMX 1.2 antibody
      • LMX-1.2 antibody
      • LMX1.2 antibody
      • LMX1B antibody
      • LMX1B_HUMAN antibody
      • MGC138325 antibody
      • MGC142051 antibody
      • NPS 1 antibody
      • NPS1 antibody
      see all

    Images

    • All lanes : Anti-LMX1b antibody (ab82835) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 56 kDa
      Observed band size : 56 kDa

    References

    ab82835 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your inquiry. I am happy to confirm that all three of the peptide sequences used for immunization in the production of the LMX1B polyclonal antibody are specific to LMX1B and therefore the antibody ab82835 should not cross react with ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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