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LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate - testosterone treated (ab14858)

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Overview

  • Product nameLNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate - testosterone treatedSee all LNCaP lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Cells were cultured in medium supplemented with 10 nM Testosterone for 6 hours at 37°C immediately prior to harvesting.

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundThe LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer.

    • References for LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate - testosterone treated (ab14858)

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"