Anti-LOX antibody (ab31238)

Phone call reporting problems in observing a clean band with ab31238.

ANSWER:

Thank you for calling us this morning.

As we discussed on the phone, I have attacheda questionnaire so thatI can gather further information regarding the samples tested and the protocol used. If you could attach an image of the results obtained that would be very useful.


OnceI havereceive the completed questionnaire,I can have a look andsee if there are any suggestionsI can make that may improve the results.


I will also look into what protocol we have used in order to perform western blotting using this antibody.

I look forward to receiving your reply.

Thank you very much for your help, I have now purchased ab27472.   In order to work out the correct dilution of antibody to use as a control for my immunohistochemistry, I believe I need to know the IgG concentration (as opposed to the protein concentration)  of the above and my 3 primary antibodies. Can you give me this information? My antibodies are as follows:   IgG ab27472 LOT: GR49002-1 s-SMA ab15734 LOT: GR17115-1 LOX ab31238 LOT: GR13790-1 LOXL2 ab96233 LOT: GR6538-6   Many Thanks  

ANSWER:

 Thank you for your patience. IgG ab27472 LOT: GR49002-1 I can confirm that the concentration is 2 ug/ml. Please do not hesitate to contact me with additional questions or concerns.  

Thank you very much for your help, I have now purchased ab27472.   In order to work out the correct dilution of antibody to use as a control for my immunohistochemistry, I believe I need to know the IgG concentration (as opposed to the protein concentration)  of the above and my 3 primary antibodies. Can you give me this information? My antibodies are as follows:   IgG ab27472 LOT: GR49002-1 s-SMA ab15734 LOT: GR17115-1 LOX ab31238 LOT: GR13790-1 LOXL2 ab96233 LOT: GR6538-6   Many Thanks  

ANSWER:

Thank you for your enquiry and your interest.

s-SMA ab15734 LOT: GR17115-1 concentration: 0.3 mg/ml LOX ab31238 LOT: GR13790-1 concentration: 1 mg/ml LOXL2 ab96233 LOT: GR6538-6 concentration: 1 mg/ml

I am contacting the Lab to find out the concentration for this particular product ( IgG ab27472 LOT: GR49002-1) and will get back to you as soon as possible.

Thank you for your patience in advance!    

Hello,   I have been doing immunohistochemistry on donkey lung tissue with a number of rabbit polyclonal antibodies and would like to purchase an isotype control to ensure that the staining I am getting is not non-specific. Can you tell me if ab27472 would be a suitable isotype control for the following antibodies?:   Anti-alpha smooth muscle Actin antibody (ab15734) Anti-LOXL2 antibody (ab96233) Anti-LOX antibody (ab31238)   I would also like to use it as a control for Millipore Anti-Tenascin AB19011 and Anti-MMP9, catalytic domain AB19016     Many Thanks  

ANSWER:

Thank you for your enquiry and interest.

Isotype controls are used to confirm that the primary antibody binding is specific and not a results of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and conjugation.

For example, if the primary antibody is a FITC-conjugated mouse IgG1, then you will need to choose a FITC-conjugated mouse IgG1 isotype control. To find a suitable isotype control on our website, go to the 'Products' section of the top navigation menu. Choose "Isotype control", then the species and subclass of your primary antibody. This will give you a range of isotype controls for use with that species and isotype of primary antibody.

Isotype controls for polyclonals: Most isotype controls are monoclonal. They are not suitable for use with polyclonal antibodies as these contain more than one IgG subclass. However, we do provide several polyclonal isotype controls for use in these circumstances. Since the isotypes of these antibodies is rabbit IgG (ab15734, ab96233, ab31238) ; ab27472 can be used as isotype control.

I hope this will be useful for you.        

Hi

Further to the e-mail below, do you know whether the antibody also binds LOXL (LOX-like) proteins?

Thanks for the information,

ANSWER:

All the testing information available for this antibody appears on the online datasheet. I would recommend checking out the images on the datasheet, Abreviews as well as references in which this antibody has been used to see if anyone has found it to react with LOX like proteins.

In addition, you might also want to allign the immunogen sequence I provided you with LOX like proteins to determine if, in theory, ab31238 may cross react with these proteins.

I hope this is helpful. Please contact me again if you have any further questions.