Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Anti-LOX antibody
See all LOX products (3) ...
Description
Rabbit polyclonal to LOX
Tested applications
WB, IHC-P, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Dog
Immunogen
Synthetic peptide derived from within residues 350 to the C-terminus of Human LOX.
(Peptide available as ab28612.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Extracellular matrix
Cardiovascular >> Vasculature >> Endothelium
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
-
Western blot - LOX antibody (ab31238)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).
IHC-P: Use at an assay dependent dilution. (PubMed: 21215756)
IHC-FoFr
IHC-FoFr: 1/300IHC-FoFr: 1/300
Target
Function
Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression.
Tissue specificity
Heart, placenta, skeletal muscle, kidney, lung and pancreas.
Involvement in disease
Defects in LOX may be a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100].
Sequence similarities
Belongs to the lysyl oxidase family.
Post-translational
modifications
The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP28300
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- lox antibody
- LYOX antibody
- LYOX_HUMAN antibody
see all
Database links
- Entrez Gene: 396474 Chicken
- Entrez Gene: 4015 Human
- Entrez Gene: 16948 Mouse
- Entrez Gene: 24914 Rat
- Omim: 153455 Human
- SwissProt: Q05063 Chicken
- SwissProt: P28300 Human
- SwissProt: P28301 Mouse
see all
Anti-LOX antibody images:
Western blot - LOX antibody (ab31238)
Anti-LOX antibody (ab31238) at 1 µg/ml + MDA-MB-361 whole cell lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 32 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-LOX antibody (ab31238)
This product has been referenced in:
- Adam Oet al. Increased lysyl oxidase expression and collagen cross-linking during atrial fibrillation. J Mol Cell Cardiol 50:678-85 (2011). WB, IHC-P; Human, Mouse, Rat.Read more (PubMed: 21215756) »
- Alarab Met al. LOX family enzymes expression in vaginal tissue of premenopausal women with severe pelvic organ prolapse. Int Urogynecol J 21:1397-404 (2010). WB, IHC-P; Human.Read more (PubMed: 20559617) »
See all 6 publications for this product
Publishing research using ab31238? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"