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Overview

  • Product nameLOX1 Mouse ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab119603 LOX1 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse LOX1 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionReceptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
  • Tissue specificityExpressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
  • Involvement in diseaseNote=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
    Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe cytoplasmic region is required for subcellular sorting on the cell surface.
    The C-type lectin domain mediates the recognition and binding of oxLDL.
  • Post-translational
    modifications    The intrachain disulfide-bonds prevent N-glycosylation at some sites.
    N-glycosylated.
  • Cellular localizationCell membrane. Secreted. A secreted form also exists.

    • Target information above from: UniProt accession P78380 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C-type lectin domain family 8 member ACLEC8AhLOX-1
        Lectin-like oxidized LDL receptor 1Lectin-like oxLDL receptor 1Lectin-type oxidized LDL receptor 1LOX-1LOXINOlr1OLR1_HUMANOx-LDL receptor 1Oxidized low density lipoprotein (lectin like) receptor 1Oxidized low density lipoprotein receptor 1 soluble formOxidized low-density lipoprotein receptor 1SCARE1Scavenger receptor class E member 1SLOX1soluble form
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119603 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      LOX1 Mouse ELISA Kit images

      • Sandwich ELISA - LOX1 Mouse ELISA Kit (ab119603)
        Sandwich ELISA - LOX1 Mouse ELISA Kit (ab119603)
        Representative standard curve using ab119603.

      Protocols

      References for LOX1 Mouse ELISA Kit (ab119603)

      ab119603 has not yet been referenced specifically in any publications.

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