Recombinant Anti-LRPAP1 antibody [EPR3327] (ab76500)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3327] to LRPAP1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LRPAP1 antibody [EPR3327]
See all LRPAP1 primary antibodies -
Description
Rabbit monoclonal [EPR3327] to LRPAP1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LRPAP1 aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: Human fetal kidney, mouse kidney and rat kidney tissue lysate and 293T cell lysates. IHC-P: Human hepatocellular carcinoma and brain tissues. IP: Human fetal kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3327 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/50 - 1/200.
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IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
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Notes |
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IP
1/50 - 1/200. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
Target
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Function
Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. -
Involvement in disease
Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis. -
Sequence similarities
Belongs to the alpha-2-MRAP family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 4043 Human
- Entrez Gene: 16976 Mouse
- Entrez Gene: 116565 Rat
- Omim: 104225 Human
- SwissProt: P30533 Human
- SwissProt: P55302 Mouse
- SwissProt: Q99068 Rat
- Unigene: 533136 Human
see all -
Alternative names
- 39 kDa receptor-associated protein antibody
- A2MRAP antibody
- A2RAP antibody
see all
Images
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All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution (unpurified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST
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All lanes : Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution (purified)
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76500 has been referenced in 1 publication.
- Marakasova E et al. Molecular chaperone RAP interacts with LRP1 in a dynamic bivalent mode and enhances folding of ligand-binding regions of other LDLR family receptors. J Biol Chem 297:100842 (2021). PubMed: 34058195