Overview

  • Product nameAnti-LRRK2 antibody [MJFF2 (c41-2)]
    See all LRRK2 primary antibodies
  • Description
    Rabbit monoclonal [MJFF2 (c41-2)] to LRRK2
  • Tested applicationsICC/IF, IHC-P, WB, IP, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HEK293 cells transfected with LRRK2
  • General notes

    Well-characterized antibodies to efficiently detect and purify LRRK2 protein are a critical need in the Parkinson's Disease (PD) research community. To help accelerate LRRK2 research, The Michael J. Fox Foundation (MJFF), working with Abcam, has generated unique and high quality LRRK2 rabbit monoclonal antibodies to be widely available for PD research community.
    LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a protein kinase belonging to the ROCO family, which is defined by the presence of a ROC (Ras/GTPase of complex proteins) domain and COR (C-terminal of Roc) region. LRRK2 exhibits kinase activity whereby it can undergo autophosphorylation and can phosphorylate generic substrates. In addition, the GTPase domain of LRRK2 can mediate GDP (guanosine-5′-diphosphate)/GTP (guanosine-5′-triphosphate) binding as well as GTP hydrolysis.

    LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients. Clinical symptoms of patients carrying PD-associated mutations of LRRK2 are indistinguishable from typical sporadic PD. The spectra of neuropathological features of PARK8 (type 8), the type corresponding to LRRK2, is broad and appears to encompass those associated with other familial PD cases such as PARK1 (alpha-synuclein) and PARK2 (Parkin). Patients with this gene mutation have typical relatively late onset Parkinsonism with features comparable with idiopathic PD; symptoms include asymmetric rest tremor, bradykinesia, rigidity, and a good response to 3,4-dihyroxy-l-phenylalanine (l-DOPA). The pathology of cases with LRRK2 mutations is pleomorphic.

    For more characterization data and protocols using this LRRK2 Antibody, please refer to Davies, et al. 2013. Biochemical J 453(1):101-113 [PMID: 23560750]

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab133474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 286 kDa.
IP Use at an assay dependent concentration. (2-5 µg)
IHC-FrFl Use at an assay dependent concentration. PubMed: 23560750
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay play a role in the phosphorylation of proteins central to Parkinson disease. Phosphorylates PRDX3. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes.
    • Tissue specificityExpressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
    • Involvement in diseaseParkinson disease 8
    • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 12 LRR (leucine-rich) repeats.
      Contains 1 protein kinase domain.
      Contains 1 Roc domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localizationCytoplasm. Membrane. Mitochondrion. Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • augmented in rheumatoid arthritis 17 antibody
      • AURA17 antibody
      • Dardarin antibody
      • Leucine rich repeat kinase 2 antibody
      • leucine rich repeat serine threonine protein kinase 2 antibody
      • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
      • LRRK 2 antibody
      • LRRK2 antibody
      • LRRK2_HUMAN antibody
      • PARK 8 antibody
      • PARK8 antibody
      • RIPK7 antibody
      • ROCO 2 antibody
      • ROCO2 antibody
      see all

    Anti-LRRK2 antibody [MJFF2 (c41-2)] images

    • Immunohistochemical analysis of mouse brain tissue, staining LRRK2 with ab133474.

      Antigen retrieval was performed by heat mediation and tissue was blocked with 2% goat serum. Sections were incubated with primary antibody (1/200) overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    • All lanes : Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab133474) at 1/10000 dilution

      Lane 1 : HEK293 cell lysate transfected with 3*Flag vector
      Lane 2 : HEK293 cell lysate transfected with 3*Flag full length wild type LRRK2

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 286 kDa

      Zhuohua Zhang Lab (Sanford-Burnham Medical Research Institute)

    References for Anti-LRRK2 antibody [MJFF2 (c41-2)] (ab133474)

    This product has been referenced in:
    • Law BM  et al. A direct interaction between leucine-rich repeat kinase 2 and specific ß-tubulin isoforms regulates tubulin acetylation. J Biol Chem 289:895-908 (2014). WB ; Mouse . Read more (PubMed: 24275654) »
    • Dorval V  et al. Gene and MicroRNA transcriptome analysis of Parkinson's related LRRK2 mouse models. PLoS One 9:e85510 (2014). WB, IP ; Mouse . Read more (PubMed: 24427314) »

    See all 18 Publications for this product

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    Merci de votre patience.


    Après vérification, mes collègues m'ont confirmé que les fiches techniques Epitomics sont exactes:

    ab133474, ab133475 et ab133476 sont des anticorps non purifiés (supern...

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    Je suis en contact avec mes collaborateurs ici à Abcam et à Epitomics afin d'éclaircir les points dont nous avons discutés par téléphone.

    Je vous contacterai de nouveau dès que j'aurai reç...

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