Anti-LRRN4CL antibody (ab188018)
Key features and details
- Rabbit polyclonal to LRRN4CL
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-LRRN4CL antibody -
Description
Rabbit polyclonal to LRRN4CL -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human LRRN4CL aa 1-150.
Database link: Q8ND94 -
Positive control
- IHC-P: Human endometrium tissue; WB: Transfected HEK-293T whole cell lysates; ICC: BJ whole cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
|
IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Sequence similarities
Contains 1 fibronectin type-III domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 100510252 Human
- Entrez Gene: 221091 Human
- SwissProt: Q8ND94 Human
- Unigene: 427449 Human
-
Alternative names
- LRN4L_HUMAN antibody
- LRRN4 C terminal like antibody
- LRRN4 C terminal like protein antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling LRRN4CL (high expression) with ab188018 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-LRRN4CL antibody (ab188018) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : LRRN4CL over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 25 kDa -
Immunocytochemical analysis of BJ (Human skin fibroblast cell line) whole cells labeling LRRN4CL in the nucleoplasm with ab188018 at 2 µg/ml.
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LRRN4CL (low expression) with ab188018 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab188018 has not yet been referenced specifically in any publications.