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Anti-LSM8 antibody [2527C6a] (ab53220)

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Overview

Product name

Anti-LSM8 antibody [2527C6a]
See all LSM8 products (2) ...

Description

Mouse monoclonal [2527C6a] to LSM8

Tested applications

WB, Dot Blotmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment of Human LSM8

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab53220 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.

Clonality

Monoclonal

Clone number

2527C6a

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

  • Western blot - LSM8 antibody [2527C6a] (ab53220)Western blot - LSM8 antibody [2527C6a] (ab53220) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 10 kDa).

  • ShowHide

    Dot Blot

     Dot: Use at an assay dep...Read more →

    Dot: Use at an assay dependent dilution.

Target

Relevance

LSM8 is a member of the LSm family and forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP. It binds specifically to the 3'-terminal U-tract of U6 snRNA.

Cellular localization

Nuclear

Alternative names

  • 2010003I05Rik antibody
  • AW214405 antibody
  • LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae) antibody
  • MGC127377 antibody
  • YJR022W antibody
see all

Database links

Anti-LSM8 antibody [2527C6a] images:

  Western blot - LSM8 antibody [2527C6a] (ab53220)

Western blot - LSM8 antibody [2527C6a] (ab53220)

Anti-LSM8 antibody [2527C6a] (ab53220) + immunised recombinant protein fragment

Predicted band size : 10 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)

References for Anti-LSM8 antibody [2527C6a] (ab53220)

ab53220 has not yet been referenced specifically in any publications.

Publishing research using ab53220? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"