Overview

  • Product nameAnti-LSS antibodySee all LSS primary antibodies ...
  • Description
    Rabbit polyclonal to LSS
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    a synthetic peptide corresponding to a region of Human LSS

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

Anti-LSS antibody images

  • Anti-LSS antibody (ab80364) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-LSS antibody (ab80364) at 1/1000 dilution + lysate prepared from murine brain.

    Predicted band size : 83 kDa

References for Anti-LSS antibody (ab80364)

This product has been referenced in:
  • Rodríguez-Penas D  et al. Aliskiren affects fatty-acid uptake and lipid-related genes in rodent and human cardiomyocytes. Biochem Pharmacol 82:491-504 (2011). WB ; Rat . Read more (PubMed: 21640714) »

See 1 Publication for this product

Product Wall

Thank you for updating me about this.

I am very sorry that this replacement p53 antibody also did not work in your experiments. I am sorry for the inconvenience and would like to offer replacement with another p53 antibody such as ab32049 or...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for this added information.

I am very sorry that these products did not work as expected. After review of the protocols and sample preparation I can see no reason why the anti p53 would not work. As such I would be very happy to re...

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Thank you for this additional information.

I have a few further questions regarding your samples that I hope you might help me with. Could you please tell me what human cell line you are using? Also how are your samples processed for the w...

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I am very sorry to hear about the troubles which you have been help. In order to fully understand the problems could you please send me your protocols which you have used, sample preparation information, any tr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"