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Western blot - LacI antibody [0.T.81] (ab33832)
![Western blot - LacI antibody [0.T.81] (ab33832)](/ps/datasheet/images/33/ab33832/LacI-Primary-antibodies-ab33832-5.jpg)
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Product Name
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LacI antibody [0.T.81]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [0.T.81] to LacI
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Immunogen
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Recombinant TrpE fusion protein containing full length E. coli LacI.
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Reacts with
(species key)
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E coli
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Specificity
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This antibody recognizes Lac1 and recombinant proteins containing a Lac1 tag.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-Fo: Use at an assay dependent dilution. The antibody shows nuclear immunostaining in Lac1 transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X100.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Lac1 transfected Cos-1 cells
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Cellular localization
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Nuclear
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Research areas
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Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 945007 (Escherichia coli )
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| SwissProt | | P03023 (Escherichia coli )
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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0.T.81
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, PBS, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.60 - 0.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LacI antibody [0.T.81] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for LacI antibody [0.T.81] images, references, products related to ab33832 and other tools.
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LacI antibody [0.T.81] images:
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 Western blot - LacI antibody [0.T.81] (ab33832)
Ab33832 (1/1000) staining Lacl in Escherichia coli cultured cell lysate
Secondary Antibody: An HRP-conjugated Donkey anti-mouse monoclonal (1/20000) Lane 1: Molecular weight Marker
Lane 2: Rainbow Molecular weight Marker
Lane 3: Blank
Lane 4: pCMV Vector cell lysate 1
Lane 5: pCMV Vector cell lysate 2 Performed under reduced and denaturing running conditions.
Blocking step: 5% Milk for 1 hour at 22°C. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to LacI
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LacI antibody [0.T.81] - more information
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Customer reviews (feedback) regarding LacI antibody [0.T.81] |
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LacI antibody [0.T.81] - related products:
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LacI antibody [0.T.81] - other tools:
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Contact Abcam with a Technical Enquiry about LacI antibody [0.T.81] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - LacI antibody [0.T.81] (ab33832)](/index.html?datasheet=33832#LacI-Primary-antibodies-ab33832-5.jpg)
