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Anti-LacI antibody [0.T.81] (ab33832)

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Overview

Product name

Anti-LacI antibody [0.T.81]

Description

Mouse monoclonal [0.T.81] to LacI

Specificity

This antibody recognizes Lac1 and recombinant proteins containing a Lac1 tag.

Tested applications

WB, ICC/IF, IHC-FoFrmore details

Cross reactivity

Reacts with

Escherichia coli

Immunogen

Recombinant TrpE fusion protein containing full length E. coli LacI.

Positive control

Lac1 transfected Cos-1 cells

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

0.T.81

Isotype

IgG1

Research areas

Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Escherichia coli

  • Western blot - LacI antibody [0.T.81] (ab33832)Western blot - LacI antibody [0.T.81] (ab33832) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at an assay dependent dilution.
IHC-Fo: Use at an assay dependent dilution. The antibody shows nuclear immunostaining in Lac1 transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X100.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Cellular localization

Nuclear

Alternative names

  • DNA binding transcriptional repressor antibody
  • LacI antibody
  • Transcriptional repressor of the lac operon antibody

Database links

Anti-LacI antibody [0.T.81] images:

  Western blot - LacI antibody [0.T.81] (ab33832)

Western blot - LacI antibody [0.T.81] (ab33832)

Ab33832 (1/1000) staining Lacl in Escherichia coli cultured cell lysate
Secondary Antibody: An HRP-conjugated Donkey anti-mouse monoclonal (1/20000)

Lane 1: Molecular weight Marker
Lane 2: Rainbow Molecular weight Marker
Lane 3: Blank
Lane 4: pCMV Vector cell lysate 1
Lane 5: pCMV Vector cell lysate 2

Performed under reduced and denaturing running conditions.
Blocking step: 5% Milk for 1 hour at 22°C.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-LacI antibody [0.T.81] (ab33832)

ab33832 has not yet been referenced specifically in any publications.

Publishing research using ab33832? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"