Overview

  • Product name
    Lactate Dehydrogenase B (LDHB) Activity Assay
    See all Lactate Dehydrogenase B kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 5%
  • Sample type
    Cell culture supernatant, Serum, Plasma, Adherent cells, Tissue Extracts, Cell Lysate, Purified protein
  • Assay type
    Enzyme activity
  • Range
    8 ng/ml - 500 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 86% - 111%
    Cell culture media 94 91% - 101%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Goat, Cow, Human
    Does not react with: Mouse, Rat
  • Product overview

    The microplate assay ab140361 is used to determine Lactate dehydrogenase B activity (LDHB) in a sample utilizing the immunocapture technique.  The specific native LDHB enzyme is immune-captured within the wells of the microplate and activity is determined by following the production of NADH catalyzed by the enzyme.  The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1 cm-1).  The immune-capture based activity assay kit contains all the required reagents for a fast and simple measurement of LDHB activity in a whole cell extract, tissue homogenate, or serum.

  • Notes

    Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDHA and LDHB genes, respectively.  The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits.  The other variants contain both types of subunits.  Elevated levels of LDH indicate tissue breakdown in hemolysis.  LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10.  Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV.  Defects in LDHB are a cause of hereditary LDHB deficiency; however LDHB deficiency is usually asymptomatic.

  • Tested applications
    Suitable for: ELISA, WBmore details
  • Platform
    Microplate

Properties

  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Alternative names
    • Epididymis secretory protein Li 281
    • HEL S 281
    • L lactate dehydrogenase B chain
    • L-lactate dehydrogenase B chain
    • Lactate dehydrogenase H chain
    • LDH B
    • LDH H
    • LDH heart subunit
    • LDH-B
    • LDH-H
    • LDHB
    • LDHB_HUMAN
    • LDHBD
    • LDHH
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY-REN-46
    • TRG-5
    • TRG5
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab140361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Lactate Dehydrogenase B (LDHB) Activity Assay images

  • LDHB activity was measured from Hela cell lysate, which contains hetero-tetramer isozyme composed of both (M) LDHA and (H) LDHB subunits. However, the liver derived cell line, HepG2, which contains mainly homo-tetramer of subunit M (LDHA), shows no enzymatic activity.
  • The species cross reactivity of this assay was demonstrated by testing the sera from multiple species. The kit shows a good reaction with human, goat and bovine samples, but shows no reaction with mouse or rat sera which may also contain LDHB. This species cross reaction specificity is due to the species specificity of the capture antibody.
  • Lane 1 - HeLa cell Lysate
    Lane 2 - HepG2 Cell Lysate
    Lane 3 - LDHA (purified from rabbit skeletal muscle)
    Lane 4 - LDHB (recombinant)
    Lane 5 - Hela – In-well extracted with capture mAb
    Lane 6 - HepG2 – In-well extracted with capture mAb
    Lane 7 - LDHA – In-well extracted with a capture mAb
    Lane 8 - LDHB – In-well extracted with capture mAb

    The western blot results indicate that the capture antibody isolates active LDHB, but does not isolate LDHA (which would be detected by the anti-LDH antibody).

Protocols

References for Lactate Dehydrogenase B (LDHB) Activity Assay (ab140361)

This product has been referenced in:
  • Song T  et al. Antibodies against Clonorchis sinensis LDH could cross-react with LDHB localizing on the plasma membrane of human hepatocarcinoma cell SMMC-7721 and induce apoptosis. Parasitol Res 115:1595-603 (2016). Read more (PubMed: 26769711) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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