![Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996) image](#Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-1.jpg)
![Flow Cytometry - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996) image](#Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-2.jpg)
![Immunoprecipitation - Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996) image](#Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-3.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Lactate Dehydrogenase B antibody [10E6AA9]
See all Lactate Dehydrogenase B products (5) ...
Description
Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B
Specificity
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
Tested applications
ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Hela cell cytosol fraction
Positive control
HeLa cells and cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
Clonality
Monoclonal
Clone number
10E6AA9
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab112996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 2 µg/ml.
IP: Use at an assay dependent concentration.
In-Cell ELISA: Use a concentration of 2 µg/ml.
Flow Cyt: Use a concentration of 1 - 2 µg/ml. Triton permeabilization required.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07195
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- L lactate dehydrogenase B chain antibody
- L-lactate dehydrogenase B chain antibody
- LDH B antibody
see all
Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
Anti-Lactate Dehydrogenase B antibody [10E6AA9] images:
Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
![Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)](/ps/datasheet/images/112/ab112996/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-1.jpg)
ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
Flow Cytometry - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
![Flow Cytometry - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)](/ps/datasheet/images/112/ab112996/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-2.jpg)
ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
Immunoprecipitation - Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
![Immunoprecipitation - Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)](/ps/datasheet/images/112/ab112996/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-3.jpg)
Isolation of LDHB using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.
References for Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
ab112996 has not yet been referenced specifically in any publications.
Publishing research using ab112996? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"