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Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

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Overview

Product name

Anti-Lactate Dehydrogenase C antibody [EP1746Y]
See all Lactate Dehydrogenase C products (5) ...

Description

Rabbit monoclonal [EP1746Y] to Lactate Dehydrogenase C

Specificity

This antibody reacts with Lactate Dehydrogenase C.

Tested applications

WB, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues on the C terminus of human Lactate Dehydrogenase C.

Positive control

WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1746Y

Isotype

IgG

  • Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/10000 - 1/20000.De...Read more →

    WB: 1/10000 - 1/20000.Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).

  • Flow Cyt

     Flow Cyt: 1/50

    Flow Cyt: 1/50

  • 1 Image

    IHC-P

     IHC-P: 1/50

    IHC-P: 1/50

Application notes

Is unsuitable for ,ICC or IP.

Target

Function

Possible role in sperm motility.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP07864 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cancer/testis antigen 32 antibody
  • CT32 antibody
  • EC 1.1.1.27 antibody
  • L lactate dehydrogenase C chain antibody
  • L-lactate dehydrogenase C chain antibody
  • LDH C antibody
  • LDH testis subunit antibody
  • LDH X antibody
  • LDH-C antibody
  • LDH-X antibody
  • LDH3 antibody
  • ldhc antibody
  • LDHC_HUMAN antibody
  • LDHX antibody
  • MGC111073 antibody
see all

Anti-Lactate Dehydrogenase C antibody [EP1746Y] images:

  Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 36 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.

References for Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

This product has been referenced in:

  • Wyns Cet al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P; Human.Read more (PubMed: 18664476) »

See 1 publication for this product

Publishing research using ab52747? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"