![Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) image](#ab52747_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) image](#ab52747_2.jpg)
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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Lactate Dehydrogenase C antibody [EP1746Y]
See all Lactate Dehydrogenase C products (5) ...
Description
Rabbit monoclonal [EP1746Y] to Lactate Dehydrogenase C
Specificity
This antibody reacts with Lactate Dehydrogenase C.
Tested applications
WB, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues on the C terminus of human Lactate Dehydrogenase C.
Positive control
WB: Human testis lysate. IHC-P: Human testis carcinoma tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1746Y
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/10000 - 1/20000.Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).
Flow Cyt
Flow Cyt: 1/50Flow Cyt: 1/50
IHC-P: 1/50
Application notes
Is unsuitable for ,ICC or IP.
Target
Function
Possible role in sperm motility.
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07864
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 32 antibody
- CT32 antibody
- EC 1.1.1.27 antibody
see all
Database links
- Entrez Gene: 3948 Human
- Omim: 150150 Human
- SwissProt: P07864 Human
- Unigene: 654377 Human
Anti-Lactate Dehydrogenase C antibody [EP1746Y] images:
Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)
![Western blot - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)](/ps/datasheet/Images/52/ab52747/ab52747_1.jpg)
Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747) at 1/20000 dilution + Human testis lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 36 kDa
Observed band size : 31 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)
![Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)](/ps/datasheet/Images/52/ab52747/ab52747_2.jpg)
Immunohistochemical staining of paraffin-embedded human testis carcinoma using ab52747 at a 1/50 dilution.
References for Anti-Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)
This product has been referenced in:
- Wyns Cet al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P; Human.Read more (PubMed: 18664476) »
See 1 publication for this product
Publishing research using ab52747? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"