Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

Product Name 

Lactate Dehydrogenase C antibody [EP1746Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1746Y] to Lactate Dehydrogenase C

Immunogen 

A synthetic peptide corresponding to residues on the C terminus of human Lactate Dehydrogenase C.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody reacts with Lactate Dehydrogenase C.

Tested applications 
(see key)

Flow Cyt, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50.
IHC-P: 1/50.
WB: 1/10000 - 1/20000. Detects a band of approximately 31 kDa (predicted molecular weight: 36 kDa).

Is unsuitable for ICC or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Human testis lysate.
IHC-P: Human testis carcinoma tissue.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1746Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for Lactate Dehydrogenase C antibody [EP1746Y] (ab52747)

  This product has been used in:

Wyns C et al. Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue. Hum Reprod 23:2402-14 (2008). IHC-P; Human. PubMed: 18664476

If you publish research using ab52747 please let us know so that we can cite the reference on this datasheet.

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