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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Lactate Dehydrogenase C antibody
See all Lactate Dehydrogenase C products (5) ...
Description
Goat polyclonal to Lactate Dehydrogenase C
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human, Pig, Cynomolgus Monkey
Immunogen
Synthetic peptide: C-KLGTDSDKEHWKNIH, corresponding to internal sequence amino acids 217-231 of Human Lactate Dehydrogenase C
C-KLGTDSDK EHWKNIH
Positive control
Mouse Testis lysate.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab63965 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/16000.
IHC: Use at an assay dependent dilution. Strong signal in seminiferous tubules of Mouse Testis.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Possible role in sperm motility.
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP07864
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 32 antibody
- CT32 antibody
- EC 1.1.1.27 antibody
see all
Database links
- Entrez Gene: 3948 Human
- Entrez Gene: 16833 Mouse
- Omim: 150150 Human
- SwissProt: P07864 Human
- SwissProt: P00342 Mouse
- Unigene: 654377 Human
- Unigene: 16563 Mouse
Anti-Lactate Dehydrogenase C antibody images:
Western blot - Lactate Dehydrogenase C antibody (ab63965)
Anti-Lactate Dehydrogenase C antibody (ab63965) at 0.03 µg/ml + Mouse Testis lysate at 35 µg/ml
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactate Dehydrogenase C antibody (ab63965)
ab63965, at a dilution of 1 µg/ml, staining Lactate Dehydrogenase C in mouse testis section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-Lactate Dehydrogenase C antibody (ab63965)
ab63965 has not yet been referenced specifically in any publications.
Publishing research using ab63965? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"