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Western blot - Lactate Dehydrogenase C antibody (ab63965)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactate Dehydrogenase C antibody (ab63965)
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Product Name
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Lactate Dehydrogenase C antibody
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See all Lactate Dehydrogenase C antibodies (5)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Lactate Dehydrogenase C
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Immunogen
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Synthetic peptide: C-KLGTDSDKEHWKNIH, corresponding to internal sequence amino acids 217-231 of Human Lactate Dehydrogenase C
BLAST 'C-KLGTDSDKEHWKNIH' with  or 
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Reacts with
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Ms
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Predicted to react
(species key)
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Hu, CynMk
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/16000.
IHC: Use at an assay dependent dilution. Strong signal in seminiferous tubules of Mouse Testis.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse Testis lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). LDHA and LDHB are expressed in most somatic tissues, while expression of LDHC is confined to the germinal epithelium of the testes.
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Lactate Dehydrogenase C antibody (ab63965) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab63965 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lactate Dehydrogenase C antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Lactate Dehydrogenase C antibody images, references, products related to ab63965 and other tools.
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Lactate Dehydrogenase C antibody images:
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 Western blot - Lactate Dehydrogenase C antibody (ab63965)
Lactate Dehydrogenase C antibody (ab63965) at 0.03 µg/ml + Mouse Testis lysate at 35 µg/ml
Predicted band size : 36 kDa
Observed band size : 36 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactate Dehydrogenase C antibody (ab63965)
ab63965, at a dilution of 1 µg/ml, staining Lactate Dehydrogenase C in mouse testis section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Search PubMed (MEDLINE) for references to Lactate Dehydrogenase C
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Lactate Dehydrogenase C antibody - more information
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Customer reviews (feedback) regarding Lactate Dehydrogenase C antibody |
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Protocols for Lactate Dehydrogenase C antibody |
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Lactate Dehydrogenase C antibody - related products:
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Lactate Dehydrogenase C antibody - other tools:
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Contact Abcam with a Technical Enquiry about Lactate Dehydrogenase C antibody |
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