Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)
- Product nameLactate Dehydrogenase (LDH) Assay Kit (Colorimetric)See all Lactate dehydrogenase kits ...
- Tests500 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 1 mU/ml
- Range1 mU/ml - 100 mU/ml
- Assay time0h 30m
- Product overview
In Abcam's LDH Assay Kit (Colorimetric), LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive.
Visit our FAQs page for tips and troubleshooting.
As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 500 tests LDH Assay Buffer NM 1 x 50ml LDH Positive Control Red 1 x 20µl LDH Substrate Mix Amber 1 vial NADH Standard Yellow 1 vial
- L lactate dehydrogenase A chainL lactate dehydrogenase B chainLactate dehydrogenase A
- Lactate dehydrogenase BLDHALDHBRenal carcinoma antigen NY REN 46Renal carcinoma antigen NY REN 59
Our Abpromise guarantee covers the use of ab102526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)
This product has been referenced in:
- Allam AB et al. Disruption of the S41 peptidase gene in mycoplasma mycoides capri impacts proteome profile, H(2)O(2) production, and sensitivity to heat shock. PLoS One 7:e51345 (2012). Functional Studies . Read more (PubMed: 23300541) »