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Lactate Dehydrogenase Isoenzyme V protein (Active) (ab77945)

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Overview

  • Product nameLactate Dehydrogenase Isoenzyme V protein (Active)See all Lactate Dehydrogenase Isoenzyme V proteins and peptides ...
  • Protein descriptionFull length native Lactate Dehydrogenase Isoenzyme V (mature form) purified from human liver. Swiss ID = P00338.
  • Expression hostNative

    • Properties

  • Purity= 100 % Ammonium Sulphate Precipitation
  • Purification notesab77945 is 100% pure as determined by electrophoresis. Contaminants: ALP: 0.002%, GOT: 0.20%, GGT: <0.001%, GPT: 0.01%.
  • Biological activityActivity: 1140 Unit/ml @ 37°C Dimension. Specific Activity: 157 Units/mg protein. One unit will catalyze the transformation of one micromole of Lactate to pyruvate at 37°C and pH 8.55
  • FormLiquid
  • Storage instructionsStore at +4°C. Do not freeze.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 50mM Tris-Chloride, 1mM EDTA, 1mM DTE, pH 8.3
  • Concentration information loading...
  • Additional notesConcentration is 1140Units/ml
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab77945 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab77945 can be used as a WB positive control in conjunction with ab85472.
    Functional Studies FuncS: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Cell proliferation-inducing gene 19 proteinL lactate dehydrogenase A chainLactate dehydrogenase 5
        Lactate dehydrogenase ALactate dehydrogenase isoenzyme 5Lactate dehydrogenase VLDH 5LDH ALDH MLDH muscle subunitLDH1LDH5LDHANY REN 59 antigenPIG 19PIG19Proliferation inducing gene 19 proteinRenal carcinoma antigen NY REN 59Renal carcinoma antigen NY-REN-59
      see all
  • RelevanceLactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • Cellular localizationCytoplasmic

    • Lactate Dehydrogenase Isoenzyme V protein (Active) images

    • Western blot - Lactate Dehydrogenase Isoenzyme V protein (Active) (ab77945)
      Western blot - Lactate Dehydrogenase Isoenzyme V protein (Active) (ab77945)
      Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml + Lactate Dehydrogenase Isoenzyme V protein (Active) (ab77945) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 12 minutes

    References for Lactate Dehydrogenase Isoenzyme V protein (Active) (ab77945)

    ab77945 has not yet been referenced specifically in any publications.

    Publishing research using ab77945 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab77945 Submit a Question for ab77945

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    sELISA with human serum samples
    will test ab85472, ab101562, ab77945 in sELISA

    Thank you for your phone call.
    As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
    DISCOUNT CODE: xxx for ab85472
    DISCOUNT CODE: xxx...

    Read More

    I recently purchased the product Lactate Dehydrogenase Isoenzyme V protein (Active) - (ab77945). Lot# GR54583-3 and GR54583-4. I just have a question about the concentration of the product. In the datasheet it said the concentration is 1140.00mg/ml and th...

    Read More

    Thank you for your enquiry. Sorry for the confusing information appearing on the datasheet for ab77945. The actual concentration is 7.5mg/ml and the product activity is 1140 units/ml. I hope this is helpful. Please contact me again if you have any further...

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    What is the units activity per ml for my lot, which is GR54583-2?

    Here is the information specific for ab77945 Lot GR54583-2:    Activity:  1,140 Unit/ml @ 37°C Dimension Protein:  7.5 mg Protein/ml (Coomassie) Specific Activity: 150 Units/mg protein One unit will catalyze the oxidation of on...

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    The enzyme came in a different vial than it normally does.. I was wondering if there is a difference between this vial and previous lots?

    Thanks for your inquiry. The new Lot GR54583-2 is at a higher concentration and therefore the 100 Unit package size will fit in a smaller vial compared to the previous lot. New Lot : concentration 1140 Units/mL = 88uL per 100 Units Old...

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    New vial has much lower activity than a previous vial from the same lot.

    Thanks for your call today and for letting us know about the problem with ab77945. As we discussed, I am sending a new vial which should arrive tomorrow. Please keep me updated about how this protein works in your assay. Also, if you have any data...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"