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In Abcam's LDH Assay Kit (Colorimetric), LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive.
Store kit unopened at at -20C. Once opened and the substrate mix and standard components are reconstituted, the solutions are stable at 4C for 1 week or at -20C for 1 month.
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As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.
|LDH Assay Buffer||NM||1 x 50ml|
|LDH Positive Control||Red||1 x 20µl|
|LDH Substrate Mix||Amber||1 vial|
|NADH Standard||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab102526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Kinetic profiles of approx 0.5 mU of a sample of pure LDH (Positive Control) and 2 μL frozen human serum using buffer as a background control.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"