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Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)

Q&A (18)Specific References (1)

Overview

  • Product nameLactate Dehydrogenase (LDH) Assay Kit (Colorimetric)See all Lactate dehydrogenase kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 mU/ml
  • Range
    1 mU/ml - 100 mU/ml
  • Assay time
    0h 30m
  • Product overview

    In Abcam's LDH Assay Kit (Colorimetric), LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceLactate dehydrogenase (LDH) is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
  • Cellular localizationCytoplasmic
    • Alternative names
        L lactate dehydrogenase A chainL lactate dehydrogenase B chainLactate dehydrogenase A
        Lactate dehydrogenase BLDHALDHBRenal carcinoma antigen NY REN 46Renal carcinoma antigen NY REN 59
      see all

    Applications

    Our Abpromise guarantee covers the use of ab102526 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) images

    • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
      Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
      NADH Standard Curve
    • Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
      Functional Studies - Lactate Dehydrogenase Colorimetric Assay Kit (ab102526)
      Time Curve

    Protocols

    References for Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)

    This product has been referenced in:
    • Allam AB  et al. Disruption of the S41 peptidase gene in mycoplasma mycoides capri impacts proteome profile, H(2)O(2) production, and sensitivity to heat shock. PLoS One 7:e51345 (2012). Functional Studies . Read more (PubMed: 23300541) »

    See 1 Publication for this product

    Publishing research using ab102526 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab102526

    Displaying 1 - 10 of 18 results for Q&A

    10
    Votes: Highest First

    I have kit for LDH # 102526 and I am trying to use it to measure the LDH on lung tissue.

    I used 50mg of tissue and homogenized in 250 μl buffer, then I spun down and used the supernatant. For my assay I diluted 1:10 the original supernatant a...

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    Hello!
    Based on the info you sent you basically diluted 1:10 once witht he original supernatant and then 1:10 again by using 5 ul plus assay buffer to make 50 ul total, so your final dilution is 1:100. Please let me know if you have any further questi...

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    LDH = (B*Sample Dilution)/((T2-T1)*V) = mU/ml

    My question is, if we use 5ul of sample diluted to 50ul then what values should we use for the sample dilution and volume? The grad student running the assay used 10 for the sample dilution and 0.005 ...

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    The dilution is the initial sample dilution. If you have 10 µl of a sample and are diluting it 100 times and then taking in 5 µl from that diluted sample into the well, your dilution factor would be 100. In this case dilution would be 1 since you are...

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    vorrei sapere se avete un kit per misurare la lattato deidrogenasi direttamente dal terreno delle cellule. Ho questo problema tecnico: le cellule sono fatte crescere su scaffold e non vorrei staccarle per ri-piastrarle in una multiwell, ma vorrei solament...

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    What I understand from your question is, that you would like a kit which measure lactate dehydrogenase produced from cells found in soil? In addition you would like to not detach the cells from the scaffold on which you are growing them to then repla...

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    two vials (LDH substrate mix and NADH standard) were empty

    Thank you for contacting us.

    The two vials are actually lysophilized products. It does not say this on the datasheet, however the protocol booklet itself (which should have come with the kit and can also be downloaded from our website) gives yo...

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    Buenas tardes,
    Nos gustaría recibir el certificado analítico según lote, del siguiente producto:- Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric); Ref: ab102526 Lot. XXXXX
    Muchas gracias y un saludo,

    Gracias por contactarnos.

    Adjunto a este mail envío el certificado de conformidad del kit ab102526.

    Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

    Buenas tardes,
    El pasado 28 de Septiembre recibí la oferta que adjunto. Se procedió a realizar el pedido de dicho producto y recibimos correctamente el kit.
    Hoy hemos intentado probar dicho kit pero hemos tenido un problema. Los pr...

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    Te pido disculpas por la espera.

    Siento mucho los inconvenientes causados por la confusión al etiquetar los componentes del kit. Estoy chequeando con mis compañeros responsables los lotes que tenemos actualmente en stock para confi...

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    Inquiry: your protocol for this assay contain wrong information. data analysis: equation is wrong! LDH activioty=Bxsample dilution/ (T2XT1)xV should be LDH activioty=Bxsample dilution/ (T2-T1)xV . T1 usually is 0! try getting a data from multiplying your ...

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    Thank you for contacting us.

    I am sorry that the equation provided in the protocol booklet of the Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric), ab102526, does contain a typing error. the equation should read T2-T1 as you have pointed out....

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    Hello: We are interested in using the colorimetric kit below for assays of culture media. Can you let us know if this kit requires that we use any special media specifically media with phenol red?
    Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) ...

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    Thanks for your enquiry regarding ab102526.

    We have no specific recommendations for what kind of media you could use with this assay. I just looked it up online and it looks like phenol containing medium having ˜10% FBS will enhance the signals....

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    Will phenol red in the culture media affect the results?

    There is a possibility that the PR in the media might interfere to some extent. To account for such interference, I would recommend using a control sample with just the plain media in replicate wells. Any signals due to any components in the media can the...

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    I want to measure LDH in primary mouse hepatocytes and serum. Is the assay kit 102526 the best choice for this? and if so is there any specific requirement regarding the cell culture media in which cells need to be grown to carry out colorimetric assays? ...

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    Thanks for your enquiry.

    ab102526 can certainly be used to determine LDH levels in your mouse cells and serum.

    You can use your normal growth medium to check for LDH levels in the growth medium. You would just need to use the DMEM w/10...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"