Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)

Overview

  • Product nameLactate Dehydrogenase (LDH) Assay Kit (Colorimetric)See all Lactate dehydrogenase kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 1 mU/ml
  • Range
    1 mU/ml - 100 mU/ml
  • Assay time
    0h 30m
  • Product overview

    In Abcam's LDH Assay Kit (Colorimetric), LDH reduces NAD to NADH, which then interacts with a specific probe to produce a color (λmax = 450 nm). The kit quantifies LDH activity in variety of biological samples such as in serum or plasma, cells, culture medium and fermentation, etc. The assay is quick, convenient, and sensitive.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    As LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has broad range of applications. The kit can detect 1-100 mU/ml of LDH directly in samples.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceLactate dehydrogenase (LDH) is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
  • Cellular localizationCytoplasmic
  • Alternative names
    • L lactate dehydrogenase A chain
    • L lactate dehydrogenase B chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase B
    • LDHA
    • LDHB
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY REN 59
    see all

Applications

Our Abpromise guarantee covers the use of ab102526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) images

  • NADH Standard Curve
  • Time Curve

Protocols

References for Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric) (ab102526)

This product has been referenced in:
  • Allam AB  et al. Disruption of the S41 peptidase gene in mycoplasma mycoides capri impacts proteome profile, H(2)O(2) production, and sensitivity to heat shock. PLoS One 7:e51345 (2012). Functional Studies . Read more (PubMed: 23300541) »

See 1 Publication for this product

Product Wall

The kit ab102526 is more suitable for measuring the LDH activity in tissue culture supernatants - It can also be used for tissue extracts.

ab65393 is only tested with cells and cell extracts - tissue extracts are yet to be tested therefore I ...

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Hello!
Based on the info you sent you basically diluted 1:10 once witht he original supernatant and then 1:10 again by using 5 ul plus assay buffer to make 50 ul total, so your final dilution is 1:100. Please let me know if you have any further que...

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The dilution is the initial sample dilution. If you have 10 µl of a sample and are diluting it 100 times and then taking in 5 µl from that diluted sample into the well, your dilution factor would be 100. In this case dilution would be 1 since you ...

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What I understand from your question is, that you would like a kit which measure lactate dehydrogenase produced from cells found in soil? In addition you would like to not detach the cells from the scaffold on which you are growing them to then re...

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Thank you for contacting us.

The two vials are actually lysophilized products. It does not say this on the datasheet, however the protocol booklet itself (which should have come with the kit and can also be downloaded from our website) gives...

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Gracias por contactarnos.

Adjunto a este mail envío el certificado de conformidad del kit ab102526.

Espero que os sea de utilidad, y en cualquier caso no dudéis en contactarnos para cualquier duda o sugerencia.

Te pido disculpas por la espera.

Siento mucho los inconvenientes causados por la confusión al etiquetar los componentes del kit. Estoy chequeando con mis compañeros responsables los lotes que tenemos actualmente en stock para co...

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Thank you for contacting us.

I am sorry that the equation provided in the protocol booklet of the Lactate Dehydrogenase (LDH) Assay Kit (Colorimetric), ab102526, does contain a typing error. the equation should read T2-T1 as you have pointed o...

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Thanks for your enquiry regarding ab102526.

We have no specific recommendations for what kind of media you could use with this assay. I just looked it up online and it looks like phenol containing medium having ˜10% FBS will enhance the signa...

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There is a possibility that the PR in the media might interfere to some extent. To account for such interference, I would recommend using a control sample with just the plain media in replicate wells. Any signals due to any components in the media can ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"