Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)

Overview

  • Product name
    Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]
    See all Lactate Dehydrogenase primary antibodies
  • Description
    Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Does not react with: Cow
  • Immunogen

    Rabbit muscle Lactate Dehydrogenase.

  • Positive control
    • Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab135396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.

Target

Images

  • All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml

    Lane 1 : Ladder
    Lane 2 : Human liver homogenate at 15 µg
    Lane 3 : Human heart homogenate at 15 µg
    Lane 4 : Rat liver homogenate at 15 µg
    Lane 5 : Rat heart homogenate at 15 µg
    Lane 6 : Mouse liver homogenate at 15 µg
    Lane 7 : Mouse heart homogenate at 15 µg
    Lane 8 : Ladder
    Lane 9 : Human fibroblast whole cell lysate at 15 µg
    Lane 10 : HeLa whole Cell Lysate at 15 µg
    Lane 11 : HepG2 whole cell lysate at 15 µg
    Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
    Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
    Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
    Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg

    Secondary
    Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size : 37 kDa
  • Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
  • Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
  • Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
  • In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.

References

This product has been referenced in:
  • Branco AF  et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). Read more (PubMed: 26121149) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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