Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)

Overview

• Product nameAnti-Lactate Dehydrogenase antibody [98A-1F9BB1]See all Lactate Dehydrogenase primary antibodies ...
• Description
  Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
• Tested applicationsWB, Flow Cyt, ICC, IP, In-Cell ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Human
  

  Does not react with

  Cow
• Immunogen

  Rabbit muscle Lactate Dehydrogenase.

• Positive controlHuman, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.357% HEPES, 0.877% Sodium chloride
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Clonality Monoclonal
• Clone number98A-1F9BB1
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Cancer
  • Tumor biomarkers
  • Enzymes
  • Lactic Dehydrogenase

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
• Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
• Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession P00338 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3945 Human
  • Entrez Gene: 3939 Human
  • Entrez Gene: 16832 Mouse
  • Entrez Gene: 16828 Mouse
  • Entrez Gene: 24534 Rat
  • Entrez Gene: 24533 Rat
  • Omim: 150000 Human
  • Omim: 150100 Human

  • SwissProt: P07195 Human
  • SwissProt: P00338 Human
  • SwissProt: P16125 Mouse
  • SwissProt: P06151 Mouse
  • SwissProt: P13491 Rabbit
  • SwissProt: P13490 Rabbit
  • SwissProt: P42123 Rat
  • SwissProt: P04642 Rat
  • Unigene: 2795 Human
  • Unigene: 29324 Mouse
  • Unigene: 107896 Rat

  see all

• Alternative names
  Cell proliferation-inducing gene 19 protein antibodyGSD11 antibodyL lactate dehydrogenase B chain antibody

  L-lactate dehydrogenase A chain antibodyLactate dehydrogenase A antibodyLactate dehydrogenase B antibodyLactate dehydrogenase H chain antibodyLactate dehydrogenase M antibodyLDH A antibodyLDH B antibodyLDH H antibodyLDH heart subunit antibodyLDH M antibodyLDH muscle subunit antibodyLDH-A antibodyLDH-M antibodyLDH1 antibodyldha antibodyLDHA_HUMAN antibodyLDHBD antibodyLDHM antibodyMS1111 antibodyPIG19 antibodyProliferation inducing gene 19 antibodyRenal carcinoma antigen NY REN 46 antibodyRenal carcinoma antigen NY-REN-59 antibodyTRG 5 antibodyTRG5 antibody

  see all

Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] images

• 

  Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml
  
  Lane 1 : Ladder
  Lane 2 : Human liver homogenate at 15 µg
  Lane 3 : Human heart homogenate at 15 µg
  Lane 4 : Rat liver homogenate at 15 µg
  Lane 5 : Rat heart homogenate at 15 µg
  Lane 6 : Mouse liver homogenate at 15 µg
  Lane 7 : Mouse heart homogenate at 15 µg
  Lane 8 : Ladder
  Lane 9 : Human fibroblast whole cell lysate at 15 µg
  Lane 10 : HeLa whole Cell Lysate at 15 µg
  Lane 11 : HepG2 whole cell lysate at 15 µg
  Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
  Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
  Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
  Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
  
  Predicted band size : 37 kDa
  
• 

  Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [MS#98A-1F9BB1] (ab135396)
  Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
• 

  Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
• 

  In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [MS#98A-1F9BB1] (ab135396)
  In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.