Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Product Name 

Lactate Dehydrogenase antibody [EP1566Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase

Immunogen 

Synthetic peptide: IHPVSTMVKGMYGIC (Human)
BLAST 'IHPVSTMVKGMYGIC' with or

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody reacts with Lactate dehydrogenase

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/80.
ICC/IF: 1/50 - 1/100.
IHC-P: 1/50 - 1/100. It is strongly recommended to perform an antigen retrieval step for an enhanced signal.
IP: 1/100.
WB: 1/100,000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver carcinoma tissue and Hela cell lysate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1566Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, 0.25mM EDTA, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

  This product has been used in:

Neves JS et al. Subcellular fractionation of human eosinophils: isolation of functional specific granules on isoosmotic density gradients. J Immunol Methods 344:64-72 (2009). WB; Human. PubMed: 19328806

If you publish research using ab52488 please let us know so that we can cite the reference on this datasheet.

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