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Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
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Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
(enlarge)


Product Name 

Lactate Dehydrogenase antibody [EP1566Y]

 

See all Lactate Dehydrogenase antibodies (10)...

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase

Immunogen 

Synthetic peptide: IHPVSTMVKGMYGIC (Human)
BLAST 'IHPVSTMVKGMYGIC' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody reacts with Lactate dehydrogenase

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/80.
ICC/IF: 1/50 - 1/100.
IHC-P: 1/50 - 1/100. It is strongly recommended to perform an antigen retrieval step for an enhanced signal.
IP: 1/100.
WB: 1/100,000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver carcinoma tissue and Hela cell lysate

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3945    (Human)
3939    (Human)
16828    (Mouse)
16832    (Mouse)
24534    (Rat)
24533    (Rat)

Omim

    

150000    (Human)
150100    (Human)

SwissProt

    

P00338    (Human)
P07195    (Human)
P16125    (Mouse)
P06151    (Mouse)
P04642    (Rat)
P42123    (Rat)

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1566Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, 0.25mM EDTA, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lactate Dehydrogenase antibody [EP1566Y] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Lactate Dehydrogenase antibody [EP1566Y] images, references, products related to ab52488 and other tools.

 

Lactate Dehydrogenase antibody [EP1566Y] images:

Product image  Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/100000 dilution + Hela cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 37 kDa
Observed band size : 37 kDa

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab52488 at a 1/50 dilution.

 

References for Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Specific publication references   This product has been used in:

Neves JS et al. Subcellular fractionation of human eosinophils: isolation of functional specific granules on isoosmotic density gradients. J Immunol Methods 344:64-72 (2009). WB; Human. PubMed: 19328806

If you publish research using ab52488 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to Lactate Dehydrogenase

 

Lactate Dehydrogenase antibody [EP1566Y] - more information

Customer reviews (feedback) regarding Lactate Dehydrogenase antibody [EP1566Y]

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Protocols for Lactate Dehydrogenase antibody [EP1566Y]

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Lactate Dehydrogenase antibody [EP1566Y] - related products:

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Loading controls

Lysates

 

Positive Controls for Lactate Dehydrogenase antibody [EP1566Y]

ab29546

  

HeLa (Human epithelial carcinoma cell line) Cytoplasmic Lysate

 

Compatible Secondaries for Lactate Dehydrogenase antibody [EP1566Y]

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Other Lactate Dehydrogenase Antibodies

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Lactate Dehydrogenase antibody [EP1566Y] - other tools:

Contact Abcam with a Technical Enquiry about Lactate Dehydrogenase antibody [EP1566Y]

 

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