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Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

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Overview

Product name

Anti-Lactate Dehydrogenase antibody [EP1566Y]
See all Lactate Dehydrogenase products (10) ...

Description

Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase

Specificity

This antibody reacts with Lactate dehydrogenase

Tested applications

ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: IHPVSTMVKGMYGIC (Human)

IHPVSTMVKG MYGIC

Positive control

Human liver carcinoma tissue and Hela cell lysate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1566Y

Isotype

IgG

  • Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody [EP1566Y] (ab52488) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

    Five stars (1 Abreview) ICC/IF: 1/50 - 1/100.

    ICC/IF: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/100,000Detects a b...Read more →

    WB: 1/100,000Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

  • IP

     IP: 1/100

    IP: 1/100

  • 1 Image

    Flow Cyt

     Flow Cyt: 1/50 - 1/80.

    Flow Cyt: 1/50 - 1/80.

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.(It ...Read more →

    IHC-P: 1/50 - 1/100.(It is strongly recommended to perform an antigen retrieval step for an enhanced signal.)

Target

Relevance

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

Cellular localization

Cytoplasmic

Anti-Lactate Dehydrogenase antibody [EP1566Y] images:

  Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Western blot - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/100000 dilution + Hela cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 37 kDa
Observed band size : 37 kDa

  Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab52488 at a 1/50 dilution.

  Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

ICC/IF image of ab52488 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52488, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry-Lactate Dehydrogenase antibody [EP1566Y](ab52488)

Flow Cytometry-Lactate Dehydrogenase antibody [EP1566Y](ab52488)

Overlay histogram showing HeLa cells stained with ab52488 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52488, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

This product has been referenced in:

  • Borde Cet al. Stepwise Release of Biologically Active HMGB1 during HSV-2 Infection. PLoS One 6:e16145 (2011). WB; Human.Read more (PubMed: 21283827) »
  • Martinet Wet al. Cell death-mediated cleavage of the attraction signal p43 in human atherosclerosis: implications for plaque destabilization. Arterioscler Thromb Vasc Biol 30:1415-22 (2010). WB; Human.Read more (PubMed: 20413730) »

See all 4 publications for this product

Publishing research using ab52488? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"