Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)

Not seeing any signal with ab7639.

ANSWER:

Thank you for calling Abcam earlier today.

I am sorry that ab7639 did not prove to be successful for you and as we discussed I am sending you ab18723 as a free of charge replacement. Your new reference number is *******.

I have also put a note in the order to ask our good out department to email you the tracking number when the product ships.

If there is anything else I can help you with, please let me know.

This product has not worked for me. There are no bands in my blot. I am confident of good transfer and loading. The product also is brown. Could you give me advice or a new antibody?

ANSWER:

Thank you for contacting Abcam today regarding the problems that you have been experiencing using ab7936. I am sorry that the product did not perform as it should have and hope that you have found useful the many things that we spoke about. Please let know if you have any questions or concerns and if there is anything else that I might do to help you.

Where did you see this protein appear on Western blots using this antibody?

ANSWER:

Thank you for your recent technical service inquiry.

According to data from our source this product will react with lactate dehydrogenase from rabbit muscle showing a 138kDa band under non-reducing conditions and a 36kDa band under reducing conditions. Although they do not have a picture available, information from a personal communication shows a 36kDa band when this product was used at 1:1000 in HeLa cell extracts. The reactivity was observed in the cytoplasmic fraction, and not in the nuclear fraction. About 30ug of lysate was loaded per lane onto a 10% gel for SDS-PAGE.

It is possible that the 55kDa band you are seeing is LDH. My recommendation would be to perform a peptide competition assay to see if the band competes out after pre-incubating with the protein. Biocompare has links to several companies that provide multiple kinds of lactate dehydrogenase:

http://www.biocompare.com/matrix.asp?catid=10080

I have added several papers to the reference section of our datasheet for ab7639 in which this antibody was used which may be useful.

Please do not hesitate to contact us if you have any other questions.

We just ordered two primary antibodies from Abcam, ab7671 and ab7639. I was wondering whether abcam has the original proteins, Sodium-Potassium ATPase and Lactate Dehydrogenase, that inteact with those two antibodies. I appreciate if you could assist us with information about where we can get Sodium-Potassium ATPase and Lactate Dehydrogenase, as we need controls when using the primary probing. I guess the best way to find out the controls is to ask Abcam about any availible information.

ANSWER:

Thank you for your email. I'm afraid that we don't sell Sodium-Potassium ATPase or Lactate Dehydrogenase. Just to let you know, you can read some feedback posted by customers who have used these antibodies in Western blotting by clicking on the reviews tab located on the online datasheets. If you have any more questions, please let me know.

To use in Western blotting, is a secondary antibody needed?

ANSWER:

Thank you for your enquiry. Since this primary antibody is directly conjugated to HRP, you don't need a secondary antibody. If you have any further questions, please contact us again.