Lactate Dehydrogenase antibody (HRP) (ab7639)

Product Name 

Lactate Dehydrogenase antibody (HRP)

Product type 

Primary antibodies

Description 

Goat polyclonal to Lactate Dehydrogenase (HRP)

Immunogen 

Full length native Lactate Dehydrogenase (purified).

Reacts with 
(species key)

Hu, Ms, Rb

Specificity 

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Tested applications 
(see key)

Dot, ELISA, IHC-P, IM, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000.
IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference).
IM: Use at an assay dependent dilution.
WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).



Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: purified enzyme from rabbit muscle (Worthington Biochemicals)
IHC-P: non-small-cell lung cancer (see Koukourakis et al)

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Conjugation notes 

Horseradish Peroxidase (HRP)

Storage buffer 

Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Purification notes 

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

10.000 mg/ml

Storage instructions 

Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

See below for Lactate Dehydrogenase antibody (HRP) images, references, products related to ab7639 and other tools.

Lactate Dehydrogenase antibody (HRP) images:

References for Lactate Dehydrogenase antibody (HRP) (ab7639)

  This product has been used in: (two most recent references)

Sidibe A et al. Integrated membrane protein analysis of mature and embryonic stem cell-derived smooth muscle cells using a novel combination of CyDye/biotin labeling. Mol Cell Proteomics 6:1788-97 (2007). WB; Mouse. PubMed: 17627936

Zwerschke W et al. Metabolic analysis of senescent human fibroblasts reveals a role for AMP in cellular senescence. Biochem J 376:403-11 (2003). PubMed: 12943534

See all 5 publication references for this product.


If you publish research using ab7639 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Lactate Dehydrogenase antibody (HRP)

Customer FAQs regarding Lactate Dehydrogenase antibody (HRP)

Protocols for Lactate Dehydrogenase antibody (HRP)

Price and availability of products related to Lactate Dehydrogenase antibody (HRP)

Lactate Dehydrogenase antibody (HRP) - related products: