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Anti-Lactate Dehydrogenase antibody (HRP)
See all Lactate Dehydrogenase products (10) ...
Goat polyclonal to Lactate Dehydrogenase (HRP)
HRP
Horseradish Peroxidase (HRP)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
IHC-P, Dot Blot, ELISA, Immunomicroscopy, WBmore details
Reacts with
Mouse, Rat, Rabbit, Human
Full length native Lactate Dehydrogenase (purified from rabbit muscle).
WB: purified enzyme from rabbit muscle (Worthington Biochemicals) IHC-P: non-small-cell lung cancer (see Koukourakis et al)
Liquid
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Polyclonal
IgG
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Our Abpromise guarantee covers the use of ab7639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000.
IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference).
IM: Use at an assay dependent dilution.
WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Cytoplasmic
Western blot - Lactate Dehydrogenase antibody (HRP) (ab7639)

All lanes : Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/2000 dilution
Lane 1 : U2OS whole cell lysate (Hu)
Lane 2 : 293T whole cell lysate (Hu)
Lane 3 : Cos-7 whole cell lysate (AGMk)
Lane 4 : CHO whole cell lysate(Hm)
Lane 5 : PC12 whole cell lysate (Rat)
Lane 6 : 3T3 whole cell lysate (Ms)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview
Western blot - Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)

Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/1000 dilution + Lactate Dehydrogenase protein (Active) (ab77857) at 0.01 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
This product has been referenced in:
See all 6 publications for this product
Publishing research using ab7639? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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All lanes : Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/2000 dilution
Lane 1 : U2OS whole cell lysate (Hu)
Lane 2 : 293T whole cell lysate (Hu)
Lane 3 : Cos-7 whole cell lysate (AGMk)
Lane 4 : CHO whole cell lysate(Hm)
Lane 5 : PC12 whole cell lysate (Rat)
Lane 6 : 3T3 whole cell lysate (Ms)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview

Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/1000 dilution + Lactate Dehydrogenase protein (Active) (ab77857) at 0.01 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
3
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