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Western blot - Lactate Dehydrogenase antibody (HRP) (ab7639)

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Product Name
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Lactate Dehydrogenase antibody (HRP)
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See all Lactate Dehydrogenase antibodies (10)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Lactate Dehydrogenase (HRP)
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Immunogen
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Full length native Lactate Dehydrogenase (purified).
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Reacts with
(species key)
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Hu, Ms, Rb
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Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
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Tested applications
(see key)
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Dot, ELISA, IHC-P, IM, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Dot: Use at an assay dependent dilution. ELISA: 1/1000 - 1/5000. IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference). IM: Use at an assay dependent dilution. WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: purified enzyme from rabbit muscle (Worthington Biochemicals) IHC-P: non-small-cell lung cancer (see Koukourakis et al)
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Conjugation notes
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Horseradish Peroxidase (HRP)
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Storage buffer
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Preservative: 0.01% Gentamicin Sulfate Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
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Purification notes
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This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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10.000 mg/ml
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Storage instructions
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Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lactate Dehydrogenase antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Lactate Dehydrogenase antibody (HRP) images, references, products related to ab7639 and other tools.
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Lactate Dehydrogenase antibody (HRP) images:
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Western blot - Lactate Dehydrogenase antibody (HRP) (ab7639)
All lanes : Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/2000 dilution
Lane 1 : U2OS whole cell lysate (Hu) Lane 2 : 293T whole cell lysate (Hu) Lane 3 : Cos-7 whole cell lysate (AGMk) Lane 4 : CHO whole cell lysate(Hm) Lane 5 : PC12 whole cell lysate (Rat) Lane 6 : 3T3 whole cell lysate (Ms)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview
See Abreview
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References for Lactate Dehydrogenase antibody (HRP) (ab7639)
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This product has been used in: (two most recent references)
Sidibe A et al. Integrated membrane protein analysis of mature and embryonic stem cell-derived smooth muscle cells using a novel combination of CyDye/biotin labeling. Mol Cell Proteomics 6:1788-97 (2007). WB; Mouse. PubMed: 17627936
Zwerschke W et al. Metabolic analysis of senescent human fibroblasts reveals a role for AMP in cellular senescence. Biochem J 376:403-11 (2003). PubMed: 12943534
See all 5 publication references for this product.
If you publish research using ab7639 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Lactate Dehydrogenase
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Lactate Dehydrogenase antibody (HRP) - more information
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