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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Lactate Dehydrogenase antibody (HRP)
See all Lactate Dehydrogenase products (10) ...
Description
Goat polyclonal to Lactate Dehydrogenase (HRP)
Conjugation
HRP
Conjugation notes
Horseradish Peroxidase (HRP)
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
Tested applications
IHC-P, Dot Blot, ELISA, Immunomicroscopy, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Immunogen
Full length native Lactate Dehydrogenase (purified from rabbit muscle).
Positive control
WB: purified enzyme from rabbit muscle (Worthington Biochemicals) IHC-P: non-small-cell lung cancer (see Koukourakis et al)
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Dot Blot
ELISA
Immunomicroscopy
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/5000.
IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference).
IM: Use at an assay dependent dilution.
WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Cellular localization
Cytoplasmic
Alternative names
- L lactate dehydrogenase A chain antibody
- L lactate dehydrogenase B chain antibody
- Lactate dehydrogenase A antibody
see all
Database links
- Entrez Gene: 3939 Human
- Entrez Gene: 3945 Human
- Entrez Gene: 16828 Mouse
- Entrez Gene: 16832 Mouse
- Entrez Gene: 24533 Rat
- Entrez Gene: 24534 Rat
- Omim: 150000 Human
- Omim: 150100 Human
see all
Anti-Lactate Dehydrogenase antibody (HRP) images:
Western blot - Lactate Dehydrogenase antibody (HRP) (ab7639)
All lanes : Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/2000 dilution
Lane 1 : U2OS whole cell lysate (Hu)
Lane 2 : 293T whole cell lysate (Hu)
Lane 3 : Cos-7 whole cell lysate (AGMk)
Lane 4 : CHO whole cell lysate(Hm)
Lane 5 : PC12 whole cell lysate (Rat)
Lane 6 : 3T3 whole cell lysate (Ms)
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an anonymous Abreview
Western blot - Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)
Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/1000 dilution + Lactate Dehydrogenase protein (Active) (ab77857) at 0.01 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)
This product has been referenced in:
- Cadete VJet al. Effect of the Rho kinase inhibitor Y-27632 on the proteome of hearts with ischemia-reperfusion injury. Proteomics : (2010). WB; Rat.Read more (PubMed: 21086464) »
- Fuster JJet al. Tumor suppressor p27(Kip1) undergoes endolysosomal degradation through its interaction with sorting nexin 6. FASEB J 24:2998-3009 (2010). WB; Mouse.Read more (PubMed: 20228253) »
See all 6 publications for this product
Publishing research using ab7639? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"