Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)

Overview

  • Product nameAnti-Lactate Dehydrogenase antibody (HRP)
    See all Lactate Dehydrogenase primary antibodies
  • Description
    Goat polyclonal to Lactate Dehydrogenase (HRP)
  • ConjugationHRP
  • Conjugation notesHorseradish Peroxidase (HRP)
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
  • Tested applicationsIHC-P, Dot Blot, ELISA, Immunomicroscopy, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Full length native Lactate Dehydrogenase (purified from rabbit muscle).

  • Positive control
    • WB: purified enzyme from rabbit muscle (Worthington Biochemicals) IHC-P: non-small-cell lung cancer (see Koukourakis et al)

Properties

Applications

Our Abpromise guarantee covers the use of ab7639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Dot Blot
ELISA
Immunomicroscopy
WB
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/5000.
    IHC-P: 1/200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference).
    IM: Use at an assay dependent dilution.
    WB: 1/1000-1/10000 (see Abreview). Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase A chain antibody
      • L lactate dehydrogenase B chain antibody
      • L-lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase B antibody
      • Lactate dehydrogenase H chain antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH A antibody
      • LDH B antibody
      • LDH B antibody
      • LDH H antibody
      • LDH H antibody
      • LDH heart subunit antibody
      • LDH heart subunit antibody
      • LDH M antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH muscle subunit antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • LDHA antibody
      • LDHA_HUMAN antibody
      • LDHB antibody
      • LDHBD antibody
      • LDHM antibody
      • MS1111 antibody
      • NY REN 46 antigen antibody
      • NY REN 59 antigen antibody
      • PIG 19 antibody
      • PIG19 antibody
      • Proliferation inducing gene 19 antibody
      • Proliferation inducing gene 19 protein antibody
      • Renal carcinoma antigen NY REN 46 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      • TRG 5 antibody
      • TRG5 antibody
      see all

    Anti-Lactate Dehydrogenase antibody (HRP) images

    • All lanes : Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/2000 dilution

      Lane 1 : U2OS whole cell lysate (Hu)
      Lane 2 : 293T whole cell lysate (Hu)
      Lane 3 : Cos-7 whole cell lysate (AGMk)
      Lane 4 : CHO whole cell lysate(Hm)
      Lane 5 : PC12 whole cell lysate (Rat)
      Lane 6 : 3T3 whole cell lysate (Ms)

      Lysates/proteins at 30 µg per lane.


      Performed under reducing conditions.

      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Anti-Lactate Dehydrogenase antibody (HRP) (ab7639) at 1/1000 dilution + Active human Lactate Dehydrogenase full length protein (ab77857) at 0.01 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Anti-Lactate Dehydrogenase antibody (HRP) (ab7639)

    This product has been referenced in:
    • Esmaeilpour T  et al. Effect of follicular fluid and platelet-activating factor on lactate dehydrogenase C expression in human asthenozoospermic samples. Iran J Med Sci 39:20-8 (2014). WB . Read more (PubMed: 24453390) »
    • Li Q  et al. Estradiol accelerates the effects of fluoxetine on serotonin 1A receptor signaling. Psychoneuroendocrinology 38:1145-57 (2013). WB ; Rat . Read more (PubMed: 23219224) »

    See all 10 Publications for this product

    Product Wall

    Thank you for your inquiry.

    We are in the process of updatingall our Safety DataSheets. We have added the SDS for ab7639 but it may take a day or two to display online (look for it under the Datasheet PDF link).

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mammary gland lysate)
    Loading amount 50 µg
    Specification mammary gland lysate
    Gel Running Conditions Reduced Denaturing (10 % polyacrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. Kristina Terrell

    Verified customer

    Submitted Sep 16 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Huh 7.5 cells)
    Loading amount 12 µg
    Specification Huh 7.5 cells
    Gel Running Conditions Reduced Denaturing (12.5% gel)
    Blocking step casein as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 27 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (U2OS and 293T cell line)
    Loading amount 30 µg
    Specification U2OS and 293T cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2007

    Thank you for your recent technical service inquiry. According to data from our source this product will react with lactate dehydrogenase from rabbit muscle showing a 138kDa band under non-reducing conditions and a 36kDa band under reducing conditio...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (human fibrocarcoma HT1080)
    Loading amount 5 µg
    Specification human fibrocarcoma HT1080
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Jul 25 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (B cells and tumor tissue)
    Loading amount 60 µg
    Specification B cells and tumor tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Username

    Dr. Jonas Nilsson

    Verified customer

    Submitted Mar 21 2006

    Thank you for your email. I'm afraid that we don't sell Sodium-Potassium ATPase or Lactate Dehydrogenase. Just to let you know, you can read some feedback posted by customers who have used these antibodies in Western blotting by clicking on the reviews...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"