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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab2101 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
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ANSWER: |
Thank you for your enquiry. I have been in touch with the source of ab2101. They have informed me that under reducing conditions a 35kDa band was observed when using rabbit muscle. It mat be that the doublet that you are observing could be tissue specific isozymes that may have slightly different structures and therefore result in different MW bands by WB. I hope this information helps. Please do not hesitate to contact me should you require further assistance. |
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I have already spoken to someone from tech services, and I was told to send an email to get the answer to my question. I have just purchased the anti-Lactate Dehydrogenase antibody (ab2101), and I need to know the expected molecular weight of the protein. Unfortuantely, this information is not indicated on the product data sheet. If someone would please reply to my e-mail, I would appreciate it. |
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ANSWER: |
Thank you for your enquiry. The antibody will react with all "H" and "M" tetrameric isozymes of LDH. The expected molecular weight of the protein is 138kDa under non-reducing conditions and 35kDa under reduced. |
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Is this antibody specific to any of the LDH isoforms (eg LDH-A, LDH-C) or isoenzymes (LDH 1-5)? |
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ANSWER: |
The antibody will react with all "H" and "M" tetrameric isozymes of LDH. If you have any more questions, please contact us again. |
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What is the molecular size of this product; lactate dehydrogenase (rabbit muscle). Please let me know as soon as possible. I want to sent out a publication by monday. |
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ANSWER: |
138kDa under non-reducing conditions. 35kDa when reduced. |
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I'd like to use antibody against LDH, but in Drosophila. Do you think antibodies from mammals could work? |
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ANSWER: |
The cross reactivity of this antibody with Drosophila has not been assessed, therefore we can't guarantee results. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
