Anti-Lactate Dehydrogenase antibody (ab2101)

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Lactate Dehydrogenase antibody (ab2101)

ab2101 staining Lactate Dehydrogenase in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). Alexa Fluor® 546 conjugated anti goat antibody was used as secondary.

Image from Hashimoto T. et. al., PLoS ONE. 2008; 3(8): e2915 (Fig. 4B)

  Immunocytochemistry/ Immunofluorescence-Lactate Dehydrogenase antibody(ab2101)

ICC/IF image of ab2101 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2101, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.