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Read our guarantee »Products:Signal Transduction >> Metabolism >> Energy Metabolism
Anti-Lactate Dehydrogenase antibody
See all Lactate Dehydrogenase products (10) ...
Mouse monoclonal to Lactate Dehydrogenase
WB, ICC/IF, IHC-Pmore details
Reacts with
Human
Recombinant full length protein, corresponding to amino acids 1-335 of Human Lactate Dehydrogenase
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
PBS, pH 7.2
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Protein G purified
Monoclonal
IgG1
kappa
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Our Abpromise guarantee covers the use of ab55433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Cytoplasmic
Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)

Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Lactate Dehydrogenase antibody (ab55433)

Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.
Western blot - Lactate Dehydrogenase antibody (ab55433)

Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This product has been referenced in:
See 1 publication for this product
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Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.

Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.

Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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