|
|
|
|
Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)
(enlarge)
|
|
IHC-P - Lactate Dehydrogenase antibody (ab55433)
(enlarge)
|
|
Western blot - Lactate Dehydrogenase antibody (ab55433)
(enlarge)
|
|
|
|
|
Product Name
|
Lactate Dehydrogenase antibody
|
| |
See all Lactate Dehydrogenase antibodies (10)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal to Lactate Dehydrogenase
|
|
Immunogen
|
Recombinant full length protein, corresponding to amino acids 1-335 of Human Lactate Dehydrogenase
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
|
|
Relevance
|
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: None
PBS, pH 7.2
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lactate Dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
Lactate Dehydrogenase antibody images:
|
 Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.
|
| |
IHC-P - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.
|
| |
Western blot - Lactate Dehydrogenase antibody (ab55433)
Predicted band size : 37 kDa
Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Lactate Dehydrogenase
|
| |
Lactate Dehydrogenase antibody - more information
|
Customer reviews (feedback) regarding Lactate Dehydrogenase antibody |
Customer FAQs regarding Lactate Dehydrogenase antibody |
Protocols for Lactate Dehydrogenase antibody |
Price and availability of products related to Lactate Dehydrogenase antibody |
| |
Lactate Dehydrogenase antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Lactate Dehydrogenase antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
