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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Anti-Lactate Dehydrogenase antibody
See all Lactate Dehydrogenase products (10) ...
Description
Mouse monoclonal to Lactate Dehydrogenase
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-335 of Human Lactate Dehydrogenase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).
Cellular localization
Cytoplasmic
Alternative names
- LDH A antibody
- LDH B antibody
- LDH H antibody
see all
Database links
- Entrez Gene: 3939 Human
- Entrez Gene: 3945 Human
- Omim: 150000 Human
- Omim: 150100 Human
- SwissProt: P00338 Human
- SwissProt: P07195 Human
Anti-Lactate Dehydrogenase antibody images:
Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.
Western blot - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-Lactate Dehydrogenase antibody (ab55433)
This product has been referenced in:
- Maxwell GLet al. Proteomic analysis of stage I endometrial cancer tissue: Identification of proteins associated with oxidative processes and inflammation. Gynecol Oncol : (2011). WB; Human.Read more (PubMed: 21458040) »
See 1 publication for this product
Publishing research using ab55433? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"