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Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)
Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)
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IHC-P - Lactate Dehydrogenase antibody (ab55433)
IHC-P - Lactate Dehydrogenase antibody (ab55433)
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Western blot - Lactate Dehydrogenase antibody (ab55433)
Western blot - Lactate Dehydrogenase antibody (ab55433)
(enlarge)


Product Name 

Lactate Dehydrogenase antibody

 

See all Lactate Dehydrogenase antibodies (10)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to Lactate Dehydrogenase

Immunogen 

Recombinant full length protein, corresponding to amino acids 1-335 of Human Lactate Dehydrogenase

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ICC/IF, IHC-P, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IF/ICC: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Tumor biomarkers >> Enzymes >> Lactic Dehydrogenase
Signal Transduction >> Metabolism >> Energy Metabolism

Relevance 

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).

 

Alternative names Lactate Dehydrogenase antibody (ab55433)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3945    (Human)
3939    (Human)

Omim

    

150100    (Human)
150000    (Human)

SwissProt

    

P00338    (Human)
P07195    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Lactate Dehydrogenase antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Lactate Dehydrogenase antibody images:

Product image  Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)

Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase antibody (ab55433)

Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.

 

Product image  IHC-P - Lactate Dehydrogenase antibody (ab55433)

IHC-P - Lactate Dehydrogenase antibody (ab55433)

Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.

 

Product image  Western blot - Lactate Dehydrogenase antibody (ab55433)

Western blot - Lactate Dehydrogenase antibody (ab55433)



Predicted band size : 37 kDa


Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.

 
 

Search PubMed (MEDLINE) for references to Lactate Dehydrogenase

 

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