Lactoferrin Human ELISA Kit (ab108882)
- Product nameLactoferrin Human ELISA KitSee all Lactoferrin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.6 ng/ml
- Range0.625 ng/ml - 40 ng/ml
= 101 %
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Lactoferrin is an 80 kDa iron-binding glycoprotein produced by many exocrine glands with a major constituent in the secondary granules of neutroplilic leukocytes. Serum Lactoferrin concentration is much higher during inflammation. Lactoferrin is known to be an immune modulator or enhancer. Lactoferrin is present in maternal milk, saliva, tears, vaginal secretions, semen, bronchoalveolar lavage fluid, and specific granules of polymorphonuclear leukocytes (PMNs).
ab108882 Lactoferrin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human plasma, serum, urine, salvia, milk and other body fluids. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Lactoferrin in less than 4 hours. A polyclonal antibody specific for Lactoferrin has been pre-coated onto a microplate. Lactoferrin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Lactoferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Lactoferrin Antibody 100x 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Lactoferrin coated Microplate 1 x 96 wells 1 unit Human Lactoferrin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Contains 2 transferrin-like domains.
- GIG12Growth inhibiting protein 12HLF2
- LactoferrinLactoferroxin-CLactotransferrinLFLTFNeutrophil lactoferrinTalalactoferrinTRFL_HUMAN
Our Abpromise guarantee covers the use of ab108882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Lactoferrin Human ELISA Kit (ab108882)
ab108882 has not yet been referenced specifically in any publications.