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Overview

  • Product nameLactoferrin Human ELISA KitSee all Lactoferrin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    = 101 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Lactoferrin is an 80 kDa iron-binding glycoprotein produced by many exocrine glands with a major constituent in the secondary granules of neutroplilic leukocytes. Serum Lactoferrin concentration is much higher during inflammation. Lactoferrin is known to be an immune modulator or enhancer. Lactoferrin is present in maternal milk, saliva, tears, vaginal secretions, semen, bronchoalveolar lavage fluid, and specific granules of polymorphonuclear leukocytes (PMNs).

    ab108882 Lactoferrin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human plasma, serum, urine, salvia, milk and other body fluids. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures Lactoferrin in less than 4 hours. A polyclonal antibody specific for Lactoferrin has been pre-coated onto a microplate. Lactoferrin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for Lactoferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        GIG12Growth inhibiting protein 12HLF2
        LactoferrinLactoferroxin-CLactotransferrinLFLTFNeutrophil lactoferrinTalalactoferrinTRFL_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108882 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Lactoferrin Human ELISA Kit images

      • Sandwich ELISA - Lactoferrin Human ELISA Kit - 1 x 96 Well Plate (ab108882)
        Sandwich ELISA - Lactoferrin Human ELISA Kit - 1 x 96 Well Plate (ab108882)

      Protocols

      References for Lactoferrin Human ELISA Kit (ab108882)

      ab108882 has not yet been referenced specifically in any publications.

      Publishing research using ab108882 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108882 Submit a Question for ab108882

      Displaying 1 - 6 of 6 results for Abreviews and Q&A

      10
      Votes: Highest First

      Inquiry: Not a problem yet, per se, but more an inquiry about the diluent. I am planning on using the lactoferrin ELISA (ab108882), on breast milk samples. The instructions state to dilute the centrifuged breast milk 1:100,000. By my calculations, this co...

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      Thanks for your enquiry.

      I did some calculations and it seems if one starts with 5 ul milk sample and dilutes it in 6 successive 10-fold dilutions, where the final dilution is 50ul of a 100,000-fold dilution, there would be sufficient buffer to ...

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      I amlooking at purchasing the lactoferrin ELISA kit with the end of this budget cycle, however will not be using it for several months. I just wanted to confirm that the shelf life is 6 months at 4 degrees C. Do you have any products with a longer shelf l...

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      Thank you for your interest. We do not have lactoferrin assay kits with longer shelf lives, but the 6 month expiration that is specifiedis very conservative. We are confidentthe kit will be effective, assuming proper storage, within that period.

      What I mean is Journal reference or a Publication that using or referring to subject Kit.

      Thank you for your enquiry.

      I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in man...

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      Hi,

      I want to consult a question about human lactoferrin elisa kit ,Code is ab108882,I want to known its cross reactivities with bovine lactoferrin

      Thank you for contacting us.

      The cross reactivity with bovine lactoferrin is less than 1%.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Do you have any information on using your Lactoferrin Human ELISA kit (ab 108882) in other species? We are conducting a study on lactoferrin in equine milk here atXXXX University and are interested in using your product. Any information that you could pro...

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      Thank you very much for your email.

      We have never tested equine samples. We have tested canine, bovine, monkey, mouse, rat, swine, and rabbit samples. See table below.

      Species % Cross Reactivity
      Canine 1%
      Bovine 0.5%
      Mon...

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      Dear Sir/Madam The customer will set ELISA application to detect the above 4 protein from human breast milk. 1. Beta-lactoglobulin : AB8226, AB8227 2. Alpha-lactalbumin : AB20212,AB112972, AB112973 3. Casein : AB47972 4. Lac...

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      Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human orthol...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"