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Overview

  • Product nameAnti-Lactoferrin antibody [2B8]
    See all Lactoferrin primary antibodies
  • Description
    Mouse monoclonal [2B8] to Lactoferrin
  • Tested applicationsSuitable for: ELISA, WB, Sandwich ELISA, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • General notes


    Can be used in the study of immunoregulation and for characterising leukaemias.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurity tested by electrophoresis.
  • Primary antibody notesCan be used in the study of immunoregulation and for characterising leukaemias.
  • ClonalityMonoclonal
  • Clone number2B8
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 77 kDa.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Lactoferrin (Biotin) (ab25811). For sandwich ELISA, use this antibody as capture at 1µg/ml with ab25811.
IHC-Fr 1/500.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody [2B8] images

  • Sandwich ELISA - Lactoferrin antibody [2B8] (ab10110)
    Sandwich ELISA - Lactoferrin antibody [2B8] (ab10110)
    Standard curve for Lactoferrin (Analyte: ab78526); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2B8] to Lactoferrin (ab10110) at 1µg/ml and Detector Antibody Rabbit polyclonal to Lactoferrin (Biotin) (ab25811) at 0.5µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Lactoferrin antibody [2B8] (ab10110)
    Immunocytochemistry/ Immunofluorescence - Lactoferrin antibody [2B8] (ab10110)
    ICC/IF image of ab10110 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10110, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactoferrin antibody [2B8] (ab10110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactoferrin antibody [2B8] (ab10110)
    Ab10110 staining human normal trachea. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom
    IHC-P image of Lactoferrin stainig on rat lung tissue sections using ab10110 (1:100). The sections were subjected to heat mediated antigen retrieval using citric acid. Non-specific protein interaction was blocked using 1% BSA at 21°C for 10 mins. The sections were then incubated with ab10110 for 16 hrs at 21°C. The secondary antibody used was Goat Polyclonal to Mouse IgG conjugated to Biotin (1:200).

    See Abreview

Protocols

References for Anti-Lactoferrin antibody [2B8] (ab10110)

This product has been referenced in:
  • Picard-Jean F  et al. The intracellular inhibition of HCV replication represents a novel mechanism of action by the innate immune Lactoferrin protein. Antiviral Res 111:13-22 (2014). Read more (PubMed: 25193851) »
  • Principe S  et al. Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine. J Proteome Res 11:2386-96 (2012). WB . Read more (PubMed: 22339264) »

See all 4 Publications for this product

Publishing research using ab10110? Please let us know so that we can cite the reference in this datasheet.

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14 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - other (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Abcam user community

Verified customer

Submitted Oct 27 2014

Western blot abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Rat Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Abcam user community

Verified customer

Submitted Oct 27 2014

Western blot abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Western blot abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hepatocytes)
Loading amount 50 µg
Specification Hepatocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
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Abcam user community

Verified customer

Submitted Dec 22 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
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Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
abreview image
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactoferrin antibody [2B8]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
abreview image
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 30 2012

I did test it on our knockout mice, which showed presence. I also ran a
purified transferrin sample on the gel, and the AB's reacted. The AB 38155
was tested, and It didnt seem to work in mouse. Also for the transferrin, a
light non spec...

Read More

Thank you for your reply.

Unfortunately, as I was mentioning in my previous email, the antibodies we have tested in mouse were raised against the full length protein. Thus, we do not know the binding sites and such the potential cross-reactivi...

Read More

Thank you for getting back to me!
Actually, I will be using it in Western Blot. I used both antibodies ab
101101 and ab77705, which both cross reacted with transferrin.
I havent tried the 3rd antibody, any help/direction would really help.

Thank you for your reply.

I was wondering how did you confirm that the lactoferrin antibody crossreacts with transferrin? The MW of both mouse proteins (serotransferrin in particular) are almost identical (78 vs 77 kDa). These cannot be distin...

Read More

Western blot abreview for Anti-Lactoferrin antibody [2B8]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Mar 05 2012

1-10 of 14 Abreviews or Q&A

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