Anti-Lactoferrin antibody [2B8] (ab10110)

Overview

• Product nameAnti-Lactoferrin antibody [2B8]See all Lactoferrin primary antibodies ...
• Description
  Mouse monoclonal [2B8] to Lactoferrin
• SpecificityCross-reacts weakly with human transferrin.
• Tested applicationsELISA, WB, Sandwich ELISA, IHC-Fr, ICC/IF, IHC-Pmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Full length native protein (purified) (Human).

• General notes

  
  

  Can be used in the study of immunoregulation and for characterising leukaemias.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityProtein G purified
• Purification notesPurity tested by electrophoresis.
• Primary antibody notes Can be used in the study of immunoregulation and for characterising leukaemias.
• Clonality Monoclonal
• Clone number2B8
• MyelomaSp2/0
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cardiovascular
  • Blood
  • Serum Proteins

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

Applications

Target

• FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
  Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
  Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
  The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
• Sequence similaritiesBelongs to the transferrin family.
  Contains 2 transferrin-like domains.
• Cellular localizationSecreted.

• Target information above from: UniProt accession P02788 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 4057 Human
  • Entrez Gene: 17002 Mouse
  • Entrez Gene: 301034 Rat
  • Omim: 150210 Human
  • SwissProt: P02788 Human
  • SwissProt: P08071 Mouse
  • Unigene: 529517 Human
  • Unigene: 282359 Mouse

  • Unigene: 218647 Rat

  see all
• Alternative names
  • CKRX antibody
  • GIG12 antibody
  • Growth inhibiting protein 12 antibody

  • HLF2 antibody
  • Homo sapiens chemokine receptor X antibody
  • Kaliocin 1 antibody
  • Lactoferrin antibody
  • Lactoferroxin A antibody
  • Lactoferroxin B antibody
  • Lactoferroxin C antibody
  • Lactoferroxin-C antibody
  • Lactotransferrin antibody
  • LF antibody
  • LTF antibody
  • Neutrophil lactoferrin antibody
  • Talalactoferrin alfa antibody
  • Talalactoferrin antibody
  • TRFL_HUMAN antibody

  see all

Anti-Lactoferrin antibody [2B8] images

• 

  Sandwich ELISA - Lactoferrin antibody [2B8] (ab10110)
  Standard curve for Lactoferrin (Analyte: ab78526); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2B8] to Lactoferrin (ab10110) at 1µg/ml and Detector Antibody Rabbit polyclonal to Lactoferrin (Biotin) (ab25811) at 0.5µg/ml.
• 

  Immunocytochemistry/ Immunofluorescence - Lactoferrin antibody [2B8] (ab10110)
  ICC/IF image of ab10110 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10110, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lactoferrin antibody [2B8] (ab10110)
  Ab10110 staining human normal trachea. Staining is localised to the cytoplasm.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)Carl Hobbs, King`s College London, United Kingdom
  IHC-P image of Lactoferrin stainig on rat lung tissue sections using ab10110 (1:100). The sections were subjected to heat mediated antigen retrieval using citric acid. Non-specific protein interaction was blocked using 1% BSA at 21°C for 10 mins. The sections were then incubated with ab10110 for 16 hrs at 21°C. The secondary antibody used was Goat Polyclonal to Mouse IgG conjugated to Biotin (1:200).

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Protocols

• Protocol Booklet