Anti-Lactoferrin antibody [2B8] (ab10110)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Lactoferrin antibody [2B8]
See all Lactoferrin primary antibodies -
DescriptionMouse monoclonal [2B8] to Lactoferrin
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, WB, Sandwich ELISA, IHC-Fr, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Full length native protein (purified) (Human).
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General notes
Can be used in the study of immunoregulation and for characterising leukaemias.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurity tested by electrophoresis.
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Primary antibody notesCan be used in the study of immunoregulation and for characterising leukaemias.
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ClonalityMonoclonal
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Clone number2B8
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MyelomaSp2/0
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab10110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 77 kDa. | |
| Sandwich ELISA | Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Lactoferrin (Biotin) (ab25811). For sandwich ELISA, use this antibody as capture at 1µg/ml with ab25811. |
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| IHC-Fr | 1/500. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 4057 Human
- Entrez Gene: 17002 Mouse
- Entrez Gene: 301034 Rat
- Omim: 150210 Human
- SwissProt: P02788 Human
- SwissProt: P08071 Mouse
- Unigene: 529517 Human
- Unigene: 282359 Mouse
see all -
Alternative names
- GIG12 antibody
- Growth inhibiting protein 12 antibody
- HLF2 antibody
see all
Images
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![Sandwich ELISA - Anti-Lactoferrin antibody [2B8] (ab10110)](http://a.abcam.com/ps/products/10/ab10110/Images/Lactoferrin-Primary-antibodies-ab10110-10.jpg)
Sandwich ELISA - Anti-Lactoferrin antibody [2B8] (ab10110)Standard curve for Lactoferrin (Analyte: ab78526); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2B8] to Lactoferrin (ab10110) at 1µg/ml and Detector Antibody Rabbit polyclonal to Lactoferrin (Biotin) (ab25811) at 0.5µg/ml. -
![Immunocytochemistry/ Immunofluorescence - Anti-Lactoferrin antibody [2B8] (ab10110)](http://a.abcam.com/ps/products/10/ab10110/Images/Lactoferrin-Primary-antibodies-ab10110-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lactoferrin antibody [2B8] (ab10110)ICC/IF image of ab10110 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10110, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)](http://a.abcam.com/ps/products/10/ab10110/Images/Lactoferrin-Primary-antibodies-ab10110-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)Ab10110 staining human normal trachea. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)](http://a.abcam.com/ps/products/10/ab10110/Images/Lactoferrin-Primary-antibodies-ab10110-29.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody [2B8] (ab10110)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of Lactoferrin stainig on rat lung tissue sections using ab10110 (1:100). The sections were subjected to heat mediated antigen retrieval using citric acid. Non-specific protein interaction was blocked using 1% BSA at 21°C for 10 mins. The sections were then incubated with ab10110 for 16 hrs at 21°C. The secondary antibody used was Goat Polyclonal to Mouse IgG conjugated to Biotin (1:200).
Protocols
References
This product has been referenced in:
- Rageh AA et al. Lactoferrin Expression in Human and Murine Ocular Tissue. Curr Eye Res 41:883-9 (2016). Read more (PubMed: 26431065) »
- Katkar GD et al. NETosis and lack of DNase activity are key factors in Echis carinatus venom-induced tissue destruction. Nat Commun 7:11361 (2016). Read more (PubMed: 27093631) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"