Western blot - Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

Predicted band size : 70 kDa

A. HT1080 cells + siRNA control sequence

B. HT1080 cells + siRNA Lamin

This picture was submitted by Andrea Robertson as part of a review of ab8984

Immunocytochemistry/ Immunofluorescence - Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

ab8984 at a 1/200 dilution staining Asynchronous and paraformaldehyde-fixed (4%) HeLa cells by immunocytochemistry. The antibody was incubated with the cells overnight and then detected using a Cy3 conjugated Goat Anti-Mouse IgG (H+L) antibody. The image shows faint, but distinct, nuclear membrane staining.

This image is courtesy of an Abreview by Kirk McManus submitted on 27 February 2006.

Western blot - Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

All lanes : Anti-Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984) at 1/1000 dilution

Lane 1 : 10ug HeLa whole cell lysate
Lane 2 : 50ug HeLa whole cell lysate
Lane 3 : 100ug HeLa whole cell lysate

Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique

Predicted band size : 70 kDa
Observed band size : 70 kDa


Exposure time : 30 seconds

This image is courtesy of an Abreview submitted by Ms Aarti Jagannath

Immunocytochemistry/ Immunofluorescence - Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

ab8984 staining dog skeletal muscle tissue cells by ICC/IF.  Cells were fixed in methanol and incubated with ab8984, diluted 1/200, for 12 hours at 4°C.  A FITC conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

Anti-Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984) at 1/100 dilution + human fibroblast at 15 µl
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 70 kDa


Lysates were prepared in RIPA buffer on ice for 30 min. They were sonicated 4 times for 5 sec prior to mixing with loading buffer.

Flow Cytometry-Lamin A + C antibody [131C3] - Nuclear Envelope Marker(ab8984)

Overlay histogram showing HeLA cells stained with ab8984 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8984, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.