Anti-Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

Phone call requesting information on how to perform WB with Anti-Lamin A + C antibody [131C3] - Nuclear Envelope Marker (ab8984)

ANSWER:

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, Ihave attachedour questionnaire so thatI can gather further information regarding the samples tested and the protocol used. OnceI have receive the completed questionnaire,I will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

I will test the antibody in Vervet monkey cells. Those cells are used in picornavirus studies. I can easily test also our lamin antibodies (lamin A, lamin C and lamin A/C) in those cells. I guess Ill get at least some ab points from those.
Ill order the Rb antibody next week.

ANSWER:

Off course abpoints will be added to your account whenever you submit a new Abreview.

Secondly, If you are planning to use abcam antibody with Vervet Monkey cells then let me know the catalogue number; I will be happy in creating a discount code if there is a significant homology between human and monkey proteins.

Please note that the discount code has to be received before the actual purchase of antibody.

I hope this will help!

One thing. I just submitted the abreview of ab8981.
Cheers,

ANSWER:

Thank you very much for submitting the Abreview.

I have cancelled the 100% Abreview discount code for ab8981. I am happy creating a new discount code for the ab you would be interested in to buy. Please be advised that the discount code can only be given if you are expecting to use the antibody in a new species or application.

If you have submitted Abreview for ab8981, you are still eligible for Abpoints.

I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

Sorry for my late response, Im travelling again.
Ok, that sounds like a good compromise. and 100% Abreview discount after testing a new application sounds reasonable. I was wondering that would you give us the 100% Abreview discount about the immunofluorescence experiments on mouse 3T3 cells using ab8981 antibody? That would be new application and new species.
Best,

ANSWER:

DISCOUNT CODE: 100%ABR-E7AAQ
Expiration date: 04-08-2012

I am very pleased to hear you would like to accept our offer and test ab8981 in [Mouse]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for {Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Hi, Could you recommend a good antibody that I could use as loading control for western blot on cell membrane fractions? Thanks

ANSWER:

Thank you for your enquiry.

Below is a selection of antibodies that are suitable for detecting the plasma membrane and nuclear envelope. They all work in western blot and I would review the datasheets to confirm which species they are tested against.

Plasma membrane
ab4645 Anti-Pma1 antibody [40B7]
www.abcam.com/4645

ab7671 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]
www.abcam.com/ab7671

ab16505 Anti-pan Cadherin antibody
www.abcam.com/ab16505

ab22744 Anti-pan Cadherin antibody [mAbcam22744]
www.abcam.com/ab22744

Nuclear envelope
ab8980 Anti-Lamin A antibody [133A2]
www.abcam.com/ab8980

ab8984 Anti-Lamin A + C antibody [131C3]
www.abcam.com/ab8984

ab16048 Anti-Lamin B1 antibody
www.abcam.com/ab16048

I hope this is useful. Just let me know if you have further questions.