Anti-Lamin A + C antibody [JOL2] (ab40567)

Western blot - Lamin A + C antibody [JOL2] (ab40567)

Anti-Lamin A + C antibody [JOL2] (ab40567) at 1/200 dilution + HeLa whole cell lysate (10ug)

Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 74 kDa
Observed band size : 64,73 kDa (why is the actual band size different from the predicted?)


Exposure time : 10 seconds

This image is courtesy of an anonymous Abreview

Flow Cytometry-Lamin A + C antibody [JOL2](ab40567)

Overlay histogram showing HeLa cells stained with ab40567 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40567, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + C antibody [JOL2] (ab40567)

ab40567 staining Lamin A + C in HeLa cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in 100% methanol, blocked with 5% serum for 30 minutes at 22°C and then incubated with ab40567 at a 1/100 dilution for 1 hour 30 minutes at 22°C. The secondary used was ab98795, donkey anti-mouse IgG - H&L (DyLight® 550), pre-adsorbed, used at a 1/200 dilution. DAPI was used to counterstain nuclei.

Image courtesy of Nir Drayman by Abreview.

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + C antibody [JOL2] (ab40567)

ab40567 staining Lamin A + C in African Green Monkey CV1 cells by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in 100% methanol, blocked with 5% serum for 30 minutes at 22ºC and then incubated with ab40567 at a 1/100 dilution for 1 hour 30 minutes at 22ºC. The secondary used was ab98795, donkey anti-mouse IgG - H&L (DyLight® 550), pre-adsorbed, used at a 1/200 dilution. DAPI was used to counterstain nuclei.

Image courtesy of Nir Drayman by Abreview.