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All lanes : Anti-Lamin A + C antibody (ab68417) at 2 µg/ml
Lane 1 : Tissue lysates prepared from Rat spleen.
Lane 2 : Whole cell lysates prepared from Human Hela cells.
Lane 3 : Whole cell lysates prepared from Human SW620 cells.
Lane 4 : Whole cell lysates prepared from Human SMMC cells.
Lysates/proteins at 50 µg per lane.
Secondary
HRP conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size : 74 kDa
ab68417 at 1µg/ml staining Lamin A+C in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by boiling. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
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