Products:Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Envelope
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I am searching for a concentration of an antibody we received the 10th of January. We contacted you by phone friday 13th, but we are still missing an answer. |
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Thank you for contacting Abcam. |
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Anti-Lamin B Receptor antibody [E398L] (ab32535) at 1/500 dilution + Jurkat cell lysate
Predicted band size : 71 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)
Ab32535, at a 1/500 dilution, staining Lamin B Receptor in HeLa cells by Immunofluorescence.
IHC image of ab32535 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32535, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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