Lamin B Receptor antibody [E398L] (ab32535)

Product Name 

Lamin B Receptor antibody [E398L]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E398L] to Lamin B Receptor

Immunogen 

A synthetic peptide corresponding to residues of human Lamin B Receptor.

Reacts with 

Hu, Rat

Does not react with 
(species key)

Ms

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/500.
IP: 1/50.
WB: 1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 71 kDa).

Is unsuitable for FACS or IHC.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IF: HeLa cells.
WB: Jurkat cell lysate.

Cellular localization 

Integral membrane protein. Nuclear envelope inner membrane.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class V >> Lamins
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Envelope

Relevance 

Lamins are nuclear membrane proteins that serve to maintain specific cellular functions, such as DNA replication and chromatin organization. Lamin B receptor (LBR) is an integral protein of the nuclear envelope inner membrane. It is phosphorylated by CDC2 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. The cleavage of lamins results in nuclear disregulation and cell death.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E398L

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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References for Lamin B Receptor antibody [E398L] (ab32535)

  This product has been used in:

Anderson DJ et al. Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo. J Cell Biol 186:183-91 (2009). WB; Human. PubMed: 19620630

If you publish research using ab32535 please let us know so that we can cite the reference on this datasheet.

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