Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)

Our customer is looking for loading control antibody which has over 60kDa size(human sample/WB). She searched ab16048 and please check this is available.
http://www.abcam.com/Lamin-B1-antibody-Nuclear-Envelope-Marker-ab16048.html

ANSWER:

As shown in the table below, to our knowledge Lamin B1 is the highest molecular weight loading control, with 66 kDa.


Loading control
Sample type
Molecular weight (kDa)
Caution

Beta Actin

Whole Cell / cytoplasmic
43
Not suitable for skeletal muscle samples. Changes in cell-growth conditions and interactions with extracellular matrix components may alter actin protein synthesis (Farmer et al, 1983)

GAPDH
Whole Cell / cytoplasmic
30-40
Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types

Tubulin
Whole Cell / cytoplasmic
55
Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs (Sangrajrang S. et al, 1998, Prasad V et al, 2000)

VDCA1/Porin
Mitochondrial
31


COXIV
Mitochondrial
16
Many proteins run at the same 16 kDa size as COXIV

Lamin B1
Nuclear
66
Not suitable for samples where the nuclear envelope is removed

TATA binding protein TBP
Nuclear
38
Not suitable for samples where DNA is removed





The link to the list of anti-Lamin B1 antibodies is : http://www.abcam.com/index.html?pageconfig=searchresults&search=Lamin%20B1&pt=1&sk=targ&sv=Lamin%20B1%20-%20Nuclear%20Envelope%20Marker&sn=Lamin%20B1%20-%20Nuclear%20Envelope%20Marker&l=1&fViewMore=1

Seeing extra bands in western blot that previous lots did not give.

ANSWER:

Thank you for calling Abcam earlier today.
I am sorry about the issues you were having with ab10648 in western blotting. As discussed, I am sending you another vial of the antibody from a different lot number to try out. Your new order number is *********.
If there is anything else I can help you with, or if you experience any other issues with the antibody, please let me know.

I will test the antibody in Vervet monkey cells. Those cells are used in picornavirus studies. I can easily test also our lamin antibodies (lamin A, lamin C and lamin A/C) in those cells. I guess Ill get at least some ab points from those.
Ill order the Rb antibody next week.

ANSWER:

Off course abpoints will be added to your account whenever you submit a new Abreview.

Secondly, If you are planning to use abcam antibody with Vervet Monkey cells then let me know the catalogue number; I will be happy in creating a discount code if there is a significant homology between human and monkey proteins.

Please note that the discount code has to be received before the actual purchase of antibody.

I hope this will help!

One thing. I just submitted the abreview of ab8981.
Cheers,

ANSWER:

Thank you very much for submitting the Abreview.

I have cancelled the 100% Abreview discount code for ab8981. I am happy creating a new discount code for the ab you would be interested in to buy. Please be advised that the discount code can only be given if you are expecting to use the antibody in a new species or application.

If you have submitted Abreview for ab8981, you are still eligible for Abpoints.

I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

Sorry for my late response, Im travelling again.
Ok, that sounds like a good compromise. and 100% Abreview discount after testing a new application sounds reasonable. I was wondering that would you give us the 100% Abreview discount about the immunofluorescence experiments on mouse 3T3 cells using ab8981 antibody? That would be new application and new species.
Best,

ANSWER:

DISCOUNT CODE: 100%ABR-E7AAQ
Expiration date: 04-08-2012

I am very pleased to hear you would like to accept our offer and test ab8981 in [Mouse]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for {Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.