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Products:Cell Biology >> Apoptosis >> Nucleus >> Lamins
Overview
Product name
Anti-Lamin B1 antibody [ZL-5]
See all Lamin B1 products (12) ...
Description
Mouse monoclonal [ZL-5] to Lamin B1
Specificity
The antibody recognizes the 68 kDa lamin B1 isoform. Reactivity with other lamin isoforms has not been detected.
Tested applications
Flow Cyt, ICC/IF, WB, IP, IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Immunogen
Recombinant human Lamin B.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.00975% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
ZL-5
Isotype
IgG1
Light chain type
kappa
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class V >> Lamins
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Envelope
Cell Biology >> Apoptosis >> Nucleus >> Lamins
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Flow Cytometry-Lamin B1 antibody [ZL-5](ab20396)
 image](/ps/datasheet/images/20/ab20396/Lamin-B1-Primary-antibodies-ab20396-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab20396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/20
ICC/IF: Use at an assay dependent concentration. (PubMed: 18798337)
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 68 kDa.
IP: Use a concentration of 5 µg/ml
IF: Use a concentration of 10 - 15 µg/ml.
ELISA: Use a concentration of 0.2 - 1 µg/ml.
Target
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in disease
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localization
Nucleus inner membrane.
Target information above from: UniProt accessionP20700
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
Database links
- Entrez Gene: 396223 Chicken
- Entrez Gene: 4001 Human
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Omim: 150340 Human
- SwissProt: P14731 Chicken
- SwissProt: P20700 Human
- SwissProt: P14733 Mouse
see all
Anti-Lamin B1 antibody [ZL-5] images:
Flow Cytometry-Lamin B1 antibody [ZL-5](ab20396)
Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-Lamin B1 antibody [ZL-5] (ab20396)
This product has been referenced in:
- Amouroux Ret al. Oxidative stress triggers the preferential assembly of base excision repair complexes on open chromatin regions. Nucleic Acids Res : (2010). WB; Human.Read more (PubMed: 20071746) »
- Azkargorta Met al. Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184-94 (2010). WB; Mouse.Read more (PubMed: 20573986) »
See all 5 publications for this product
Publishing research using ab20396? Please let us know so that we can cite the reference in this datasheet
![Lamin B1 antibody [ZL-5] for Immunoprecipitation in Human (20396)](/ps/datasheet/images/20/ab20396/Lamin-B1-Primary-antibodies-ab20396-28.png)
![Lamin B1 antibody [ZL-5] for WB in Mouse (20396)](/ps/Reviews/Images/ab7449_31149.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"